A recombinant E0 gene of bovine viral diarrhea virus protects against challenge with bovine viral diarrhea virus of sika deer

被引：0
|
作者：
Gao, Yugang [2 ]
Wang, Yajun [3 ]
Du, Rui [2 ]
Wang, Quankai [2 ]
Zhang, Pengju [1 ]
Wang, Shuzhi [2 ]
Zhang, Lianxue [2 ]
机构：
[1] Sichuan Univ, Sch Life Sci, Chengdu 610064, Peoples R China
[2] Jinlin Agr Univ, Coll Tradit Mat Med, Changchun 130118, Peoples R China
[3] Jinlin Agr Univ, Coll Hort, Changchun 130118, Peoples R China
来源：
AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH | 2011年 / 5卷 / 09期
基金：
中国博士后科学基金; 中国国家自然科学基金;
关键词：
Sika deer; bovine viral diarrhea virus; E-0; gene; eukaryotic expression; immune response; MUCOSAL DISEASE VIRUS; PERSISTENT INFECTION; PLASMID DNA; PESTIVIRUS; GLYCOPROTEINS; PROTEINS; CELLS;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Eukaryotic expression plasmid (PVAX 1/E-0) of E-0 gene was constructed from an isolated strain of bovine viral diarrhea virus (BVDV) from sika deer and transfected into the BHK-21 cells to detect E-0 gene transcription expression level and protein expression level. It was found that E-0 gene could express in BHK-21 cells by RT-PCR and ELESA results. The humoral and cellular immune response levels were detected in rabbits immunized with PAVX1/E-0 and inactivated vaccine by ELESA technology, which reached the peak after 42 days immunization and PVAX1/E-0 immune rabbits could produce specific higher cells immune responses level in comparison to inactivated vaccine group. This is the first report demonstrating and "immunogenicity" of BVDV E-0 gene on "sika deer", indicating a new vaccine target for BVDV.
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页码：1012 / 1017
页数：6
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