Objective: To study the psychological tension in electroencephalogram (EEG) of pilots selection in order to provide the evidence for obtaining accurate and reliable results. Methods: A total of 250 senior high school students, aged 17-21 years old, were randomly selected to take part in the physical examination of pilot selection from 2019 to 2020, and were divided into the non-teaching group and the education group (self control), respectively. In the process of EEG examination, the students' comprehension, cooperation and physiological indicators were evaluated through video demonstration and teaching before examination and heart rate monitoring during examination. Results: Compared with the non-teaching group, the video demonstration helps the participating students to understand the inspection items, get familiar with the inspection process, and get good cooperation. After the beginning of the examination, the heart rate of the education group did not change significantly, and remained at the same level until the end of the examination. There was no significant difference between the heart rate after the examination and the heart rate during the examination (P > 0.05). The heart rate of the uneducated group increased and remained at the same level until the end of the examination, which was significantly different from that of the basal heart rate (P < 0.05). Conclusion: Video education and heart rate detection can effectively improve the students' understanding and cooperation on the examination items, reduce psychological tension and fear, and enhance the reliability and effectiveness of the selection results.