MOLECULAR TYPING OF Escherichia coli STRAINS ISOLATED FROM FOOD IN CASABLANCA (MOROCCO)

被引：2

作者：

Badri, S. ^{[1
]}

Fassouane, A.

Filliol, I ^{[2
]}

Hassar, M. ^{[1
]}

Cohen, N. ^{[1
]}

机构：

[1] Inst Pasteur Maroc, Lab Microbiol & Hyg Aliments & Environm, Casablanca, Morocco

[2] Inst Pasteur Paris, Ctr Natl Reference Escherichia Coli & Shigella, Unite Biodivers Bacteries Pathogenes Emergentes, Paris, France

来源：

CELLULAR AND MOLECULAR BIOLOGY | 2009年 / 55卷

关键词：

E; coli; Molecular typing; PCR-RFLP; Food; Morocco; ANTIGEN GENE-CLUSTER; VIRULENCE GENES; IDENTIFICATION; RESTRICTION; SEROGROUPS; PIGS;

D O I：

10.1170/129

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Serotyping of O- and H-antigens is regarded as the gold standard in classification of E. coli for taxonomic and epidemiological purposes similar to the Kaufmann-White scheme for Salmonella enterica. Molecular methods to replace or to support the serotyping were recently applied. Using the molecular polymorphism of the somatic (O-antigen) gene rfb cluster and flagella (H-antigen) gene fliC, 74 E. coli strains carrying the virulent genes isolated from food were characterised by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results showed that 49 (66%) of the isolates revealed a reproducible and clear cut classification, with a very good correlation to the collection of reference strains, were found.

引用

页码：1132 / 1137

页数：6

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