The quadrupole mode frequency, the monopole mode frequency, and the critical rotational frequency for stirring a single vortex nucleation along the BEC-BCS crossover are obtained. The results show that, in a rotating anisotropic an harmonic trap, the quadrupole mode frequency and the critical rotational frequency for stirring a single vortex nucleation are modified significantly when the system crosses from the BEC side to the BCS side: the anisotropy of the trap induces a downshift of the quadrupole mode frequency and the critical rotational frequency and helps the vortex formation in the system, while an anharmonic trap induces an upshift of the quadrupole mode frequency and the critical rotational frequency and suppresses the vortex formation in the system.