Single-molecule fluorescence spectroscopy of protein folding

被引:123
|
作者
Schuler, B [1 ]
机构
[1] Univ Zurich, Dept Biochem, CH-8057 Zurich, Switzerland
关键词
emission spectroscopy; FRET (fluorescence resonance energy transfer); protein folding; proteins; single-molecule studies;
D O I
10.1002/cphc.200400609
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Single-molecule spectroscopy is an important new approach for studying the intrinsically heterogeneous process of protein folding. This Review illustrates how different single-molecule fluorescence techniques have improved our understanding of mechanistic aspects in protein folding, exemplified by a series of recent experiments on a small protein.
引用
收藏
页码:1206 / 1220
页数:15
相关论文
共 50 条
  • [2] Single-molecule fluorescence spectroscopy maps the folding landscape of a large protein
    Pirchi, Menahem
    Ziv, Guy
    Riven, Inbal
    Cohen, Sharona Sedghani
    Zohar, Nir
    Barak, Yoav
    Haran, Gilad
    [J]. NATURE COMMUNICATIONS, 2011, 2
  • [3] Single-molecule fluorescence spectroscopy maps the folding landscape of a large protein
    Menahem Pirchi
    Guy Ziv
    Inbal Riven
    Sharona Sedghani Cohen
    Nir Zohar
    Yoav Barak
    Gilad Haran
    [J]. Nature Communications, 2
  • [4] Single-molecule fluorescence studies of protein folding
    Haran, Gilad
    [J]. PROTEIN SCIENCE, 2012, 21 : 66 - 66
  • [5] Single-Molecule Force Spectroscopy of Protein Folding
    Petrosyan, Rafayel
    Narayan, Abhishek
    Woodside, Michael T.
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2021, 433 (20)
  • [6] Single-Molecule Fluorescence Studies of Fast Protein Folding
    Wang, Z.
    Campos, L. A.
    Munoz, V.
    [J]. SINGLE-MOLECULE ENZYMOLOGY: FLUORESCENCE-BASED AND HIGH-THROUGHPUT METHODS, 2016, 581 : 417 - 459
  • [7] Single-Molecule Force Spectroscopy of Membrane Protein Folding
    Wijesinghe, W. C. Bhashini
    Min, Duyoung
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2023, 435 (11)
  • [8] Single-molecule spectroscopy of protein folding in a chaperonin cage
    Hofmann, Hagen
    Hillger, Frank
    Pfeil, Shawn H.
    Hoffmann, Armin
    Streich, Daniel
    Haenni, Dominik
    Nettels, Daniel
    Lipman, Everett A.
    Schuler, Benjamin
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (26) : 11793 - 11798
  • [9] Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy
    Benjamin Schuler
    Everett A. Lipman
    William A. Eaton
    [J]. Nature, 2002, 419 : 743 - 747
  • [10] Probing the free-energy surface for protein folding with single-molecule fluorescence spectroscopy
    Schuler, B
    Lipman, EA
    Eaton, WA
    [J]. NATURE, 2002, 419 (6908) : 743 - 747