RBD-Modified Bacterial Vesicles Elicited Potential Protective Immunity against SARS-CoV-2

被引：25

作者：

Yang, Zhongqian ^{[1
]}

Hua, Liangqun ^{[1
,2
]}

Yang, Mengli ^{[3
]}

Liu, Shu-Qun ^{[2
,4
,5
]}

Shen, Jianxin ^{[2
,4
,5
]}

Li, Weiran ^{[1
]}

Long, Qiong ^{[1
]}

Bai, Hongmei ^{[1
]}

Yang, Xu ^{[1
]}

Ren, Zhaoling ^{[6
,7
]}

Zheng, Xiao ^{[1
,2
]}

Sun, Wenjia ^{[1
]}

Ye, Chao ^{[1
]}

Li, Duo ^{[1
,8
]}

Zheng, Peng ^{[1
]}

He, Jinrong ^{[1
,7
]}

Chen, Yongjun ^{[1
]}

Huang, Weiwei ^{[1
]}

Peng, Xiaozhong ^{[3
,9
]}

Ma, Yanbing ^{[1
]}

机构：

[1] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biol, Lab Mol Immunol, Kunming, Yunnan, Peoples R China

[2] Yunnan Univ, Kunming, Yunnan, Peoples R China

[3] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biol, Natl Kunming High Level Biosafety Primate Res Ctr, Kunming, Yunnan, Peoples R China

[4] Yunnan Univ, State Key Lab Conservat & Utilizat Bioresources Y, Kunming, Yunnan, Peoples R China

[5] Yunnan Univ, Sch Life Sci, Kunming, Yunnan, Peoples R China

[6] Kunming Med Univ, Affiliated Hosp 2, Kunming, Yunnan, Peoples R China

[7] Kunming Med Univ, Kunming, Yunnan, Peoples R China

[8] Yunnan Prov Ctr Dis Control & Prevent, Dept Acute Infect Dis Control & Prevent, Kunming, Yunnan, Peoples R China

[9] Chinese Acad Med Sci, Peking Union Med Coll, State Key Lab Med Mol Biol,Neurosci Ctr,Sch Basic, Dept Mol Biol & Biochem,Inst Basic Med Sci,Med Pr, Beijing, Peoples R China

来源：

NANO LETTERS | 2021年 / 21卷 / 14期

基金：

中国国家自然科学基金;

关键词：

Bacterial vesicles; receptor binding domain; SARS-CoV-2; vaccine; COVID-19; OUTER-MEMBRANE VESICLES; CELLULAR-IMMUNITY; VACCINES; MATURATION;

D O I：

10.1021/acs.nanolett.1c00680

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The disease caused by SARS-CoV-2 infection threatens human health. In this study, we used high-pressure homogenization technology not only to efficiently drive the bacterial membrane to produce artificial vesicles but also to force the fusion protein ClyA-receptor binding domain (RBD) to pass through gaps in the bacterial membrane to increase the contact between ClyA-RBD and the membrane. Therefore, the load of ClyA-RBD on the membrane is substantially increased. Using this technology, we constructed a "ring-like" bacterial biomimetic vesicle (BBV) loaded with polymerized RBD (RBD-BBV). RBD-BBVs injected subcutaneously can accumulate in lymph nodes, promote antigen uptake and processing, and elicit SARS-CoV-2-specific humoral and cellular immune responses in mice. In conclusion, we evaluated the potential of this novel bacterial vesicle as a vaccine delivery system and provided a new idea for the development of SARS-CoV-2 vaccines.

引用

页码：5920 / 5930

页数：11

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