Two novel gastric cancer-associated genes identified by differential display

被引：4
|
作者：
You, H [1 ]
Xiao, B [1 ]
Cui, DX [1 ]
Shi, YQ [1 ]
Fan, DM [1 ]
机构：
[1] Fourth Mil Med Univ, Dept Gastroenterol, Xijing Hosp, Xian 710032, Peoples R China
来源：
WORLD JOURNAL OF GASTROENTEROLOGY | 1998年 / 4卷 / 04期
关键词：
stomach neoplasms; gene clone; nucleotides sequence analysis; in situ hybridization; polymerase chain reaction; RNA; messenger;
D O I：
10.3748/wjg.v4.i4.334
中图分类号：
R57 [消化系及腹部疾病];
学科分类号：
摘要：
AIM To clone novel gastric cancer-associated genes and investigate their roles in gastric cancer occurrence. METHODS A method called differential display was used which allows the identification of differentially expressed genes by using PAGE To display PCR-amplified cDNA fragments between gastric cancer cells and normal gastric mucosa cells. These fragments were cloned into plasmid vector pUC18. Homology analysis was made after sequencing these fragments. RESULTS Two novel genes were identified compared with sequences from GenBank. One was registered with the AD number AF 051783, In situ hybridization showed that these two novel genes expressed specifically in gastric cancer tissues. CONCLUSION The two novel genes obtained by differential display were confirmed to be gastric cancer-associated genes using in situ hybridization.
引用
收藏
页码：334 / 336
页数：3
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