Gene expression profiles in the liver and kidney of metallothionein-null mice

被引：21
|
作者：
Miura, N [1 ]
Koizumi, S [1 ]
机构：
[1] Natl Inst Ind Hlth, Div Hlth Effects Res, Tama Ku, Kawasaki, Kanagawa 2148585, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 332卷 / 04期
关键词：
cDNA microarray; energy metabolism; knockout mouse; metallothionein; obesity;
D O I：
10.1016/j.bbrc.2005.05.043
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
It has been reported that the expression of certain genes was altered in rodent cells lacking metal lothioneins (MTs). To further explore the effects of MT deficiency, we screened genes differentially expressed in the liver and kidney of MT-null mice by cDNA microarray analysis. In the liver, 29 of 8737 genes analyzed were altered in their expression levels: 19 and 10 genes were up-regulated and down-regulated, respectively. Particularly, 14 of the 29 genes were related to energy metabolism, and some of these suggested that loss of MTs might lead to obesity and irregular ATP synthesis. In the kidney, 41 differentially expressed genes were observed: 27 and 14 genes were up-regulated and down-regulated, respectively. Eleven of the 41 genes were also related to energy metabolism. Microarray results were confirmed by Northern blot analysis for five of the energy metabolism-related genes. (c) 2005 Elsevier Inc. All rights reserved.
引用
收藏
页码：949 / 955
页数：7
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