Design and applications of rapid-scan spectrally resolved fluorescence microscopy

被引：9
|
作者：
Haralampus-Grynaviski, NM
Stimson, MJ
Simon, JD [1 ]
机构：
[1] Duke Univ, Dept Chem, Durham, NC 27708 USA
[2] Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA
来源：
APPLIED SPECTROSCOPY | 2000年 / 54卷 / 12期
关键词：
confocal spectral imaging; fluorescence microscopy;
D O I：
10.1366/0003702001948989
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
This paper addresses the design and applications of a spectrally resolved confocal fluorescence microscope that measures spectral information for each pixel in the image with a fast multi-point approach. The combined spatial and spectral resolution of the device is demonstrated by performing two-photon imaging of mixtures of fluorescently labeled microspheres. Overlapping emission spectra of dye solutions contained in microcapillaries are readily distinguished. Images of a human skin tissue section and a mouse kidney section are presented which demonstrate that the structure and spectra of biologic samples can be resolved.
引用
收藏
页码：1727 / 1733
页数：7
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