Optimized Method for Determining Free L-Cysteine in Rat Plasma by High-Performance Liquid Chromatography with the 4-Aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole Conversion Reagent

被引：5

作者：

Otani, Lila ^{[1
]}

Ogawa, Shinya ^{[2
]}

Zhao, Zhilei ^{[1
]}

Nakazawa, Kyoko ^{[1
]}

Umehara, Shunsuke ^{[3
]}

Yoshimura, Etsuro ^{[2
]}

Chang, Sue-Joan ^{[1
,4
]}

Kato, Hisanori ^{[1
]}

机构：

[1] Univ Tokyo, Org Interdisciplinary Res Projects, Bunkyo Ku, Tokyo 1138657, Japan

[2] Univ Tokyo, Dept Appl Biol Chem, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan

[3] Prot Chem Co Ltd, Chiyoda Ku, Tokyo 1000011, Japan

[4] Natl Cheng Kung Univ, Dept Life Sci, Taipei 701, Taiwan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2011年 / 75卷 / 11期

关键词：

cysteine; cystine; reduced thiol; 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole; rat plasma; GLUTATHIONE DISULFIDE; TOTAL HOMOCYSTEINE; OXIDATIVE STRESS; THIOLS; CYSTEINYLGLYCINE; DERIVATIZATION; METHIONINE; BLOOD;

D O I：

10.1271/bbb.110356

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The analytical method was optimized for L-cysteine (Cys) in rat plasma with co-existing L-cystine (Cyss). We observed that more than 100% Cyss in rat plasma was converted to Cys under typical conditions for the conversion with 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate (SBD-F). Another conversion reagent, 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole (ABD-F), was then employed, with which the reaction could be carried out at a low temperature without the use of a reducing reagent. Under the optimized conditions of 4 degrees C and pH 8.3, the conversion ratio of Cyss to Cys in rat plasma was as low as 5-7%. We determined the Cys concentration in plasma of the portal vein of rats that had been orally administered with Cys and Cyss by applying this method. The result indicated that Cys administration and also Cyss administration effectively increased the plasma Cys level. The method developed in this study is well suited for determining the thiol compounds in biological samples.

引用

页码：2119 / 2124

页数：6

共 48 条

[31] PRECOLUMN FLUORESCENCE TAGGING REAGENT FOR CARBOXYLIC-ACIDS IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - 4-SUBSTITUTED-7-AMINOALKYLAMINO-2,1,3-BENZOXADIAZOLES
TOYOOKA, T
ISHIBASHI, M
TAKEDA, Y
NAKASHIMA, K
AKIYAMA, S
UZU, S
IMAI, K
JOURNAL OF CHROMATOGRAPHY, 1991, 588 (1-2): : 61 - 71
[32] High-performance liquid chromatographic determination of erdosteine and its optical active metabolite utilizing a fluorescent chiral tagging reagent, R-(-)-4-(N,N-dimethylaminosulfonyl)-7-(3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole
Muramatsu, N
Toyo'oka, T
Yamaguchi, K
Kobayashi, S
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1998, 719 (1-2): : 177 - 189
[33] High-performance liquid chromatographic determination of erdosteine and its optical active metabolite utilizing a fluorescent chiral tagging reagent, R-(-)-4-(N,N-dimethylaminosulfonyl)-7- (3-aminopyrrolidin-1-yl)-2,1,3-benzoxadiazole
Muramatsu, Norikazu
Toyo'oka, Toshimasa
Yamaguchi, Kazuo
Kobayashi, Satoshi
Journal of Chromatography B, 1998, 719 (1-2): : 177 - 189
[34] High-Performance Liquid Chromatography and Fluorometric Detection of Arachidonlyethanolamide (Anandamide) and its Analogs, Derivatized with 4-(N-Chloroformylmethyl-N-Methyl)Amino-7- @N,N- Dimethylaminosulphonyl-2,1,3-Benzoxadiazole (DBD-COCI)
Koga, D.
Santa, T.
Hagiwara, K.
Imai, K.
International Sugar Journal, 1995, 978
[35] Simple determination of o-phenylphenol in skin lotion by high-performance liquid chromatography coupled with fluorescence detection after pre-column derivatization with 4-(N-chloroformylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole
Higashi, Yasuhiko
Konno, Kazunori
JOURNAL OF COSMETIC SCIENCE, 2015, 66 (02) : 129 - 137
[36] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND FLUOROMETRIC DETECTION OF ARACHIDONYLETHANOLAMIDE (ANANDAMIDE) AND ITS ANALOGS, DERIVATIZED WITH 4-(N-CHLOROFORMYLMETHYL-N-METHYL)AMINO-7-N,N-DIMETHYLAMINOSULPHONYL-2,1,3-BENZOXADIAZOLE (DBD-COCL)
KOGA, D
SANTA, T
HAGIWARA, K
IMAI, K
TAKIZAWA, H
NAGANO, T
HIROBE, M
OGAWA, M
SATO, T
INOUE, K
KUDO, I
BIOMEDICAL CHROMATOGRAPHY, 1995, 9 (01) : 56 - 57
[37] Determination of plasma total homocysteine and cysteine using HPLC with fluorescence detection and an ammonium 7-fluoro-2,1,3-benzoxadiazole-4-sulphonate (SBD-F) derivatization protocol optimized for antioxidant concentration, derivatization reagent concentration, temperature and matrix pH
Daskalakis, I
Lucock, MD
Anderson, A
Wild, J
Schorah, CJ
Levene, MI
BIOMEDICAL CHROMATOGRAPHY, 1996, 10 (05) : 205 - 212
[38] High-performance liquid chromatographic assay of NG-monomethyl-L-arginine, NG-dimethyl-L-arginine, and NGNG-dimethyl-L-arginine using 4-fluoro-7-nitro-2,1,3-benzoxaldiazole as a fluorescent reagent
Nonaka, S
Tsunoda, M
Imai, K
Funatsu, T
JOURNAL OF CHROMATOGRAPHY A, 2005, 1066 (1-2) : 41 - 45
[39] Synthesis of the isotope-labeled derivatization reagent for carboxylic acidsr 7-(N,N-dimethylaminosulfonyl)-4-(aminoethyl)piperazino-2,1,3-benzoxadiazole (d6) [DBD-PZ-NH2 (D)], and its application to the quantification and the determination of relative amount of fatty acids in rat plasma samples by high-performance liquid chromatography/mass spectrometry
Tsukamoto, Y
Santa, T
Yoshida, H
Miyano, H
Fukushima, T
Hirayama, K
Imai, K
Funatsu, T
BIOMEDICAL CHROMATOGRAPHY, 2006, 20 (04) : 358 - 364
[40] FLUOROMETRIC-DETERMINATION OF PHENYLPROPANOLAMINE IN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING 4-FLUORO-7-NITROBENZO-2-OXA-1,3-DIAZOLE AS A PRECOLUMN DERIVATIZATION REAGENT
SUZUKI, H
FUJIWARA, S
AKIMOTO, K
SUGIMOTO, I
BUNSEKI KAGAKU, 1986, 35 (03) : 283 - 287

← 1 2 3 4 5 →