Lycium barbarum polysaccharide improves the development of mouse oocytes vitrified at the germinal vesicle stage

被引：17
|
作者：
Yang, Lei [1 ,3 ]
Lei, Lanjie [2 ]
Zhao, Qihan [1 ]
Gao, Zhen [4 ]
Xu, Xiaoyuan [1 ,3 ]
机构：
[1] Jiujiang Univ, Coll Basic Med Sci, Jiujiang 332000, Jiangxi, Peoples R China
[2] Jiujiang Univ, Affiliated Hosp, Jiujiang 332000, Jiangxi, Peoples R China
[3] Jiujiang Univ, Key Lab Syst Biomed Jiangxi Prov, Jiujiang 332000, Jiangxi, Peoples R China
[4] Northwest A&F Univ, Minist Agr, Key Lab Anim Biotechnol, Yangling 712100, Shaanxi, Peoples R China
来源：
CRYOBIOLOGY | 2018年 / 85卷
关键词：
Lycium barbarum polysaccharide; Endoplasmic reticulum stress; AKT; MAPK3/1; Vitrification; VITRIFICATION; MATURATION; ACTIVATION; EXPRESSION;
D O I：
10.1016/j.cryobiol.2018.10.265
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The cryopreservation of cumulus oocyte complexes (COCs) is an important process in reproductive medicine. However, this process slows both embryo and offspring development. In this study, the effects of maturation medium supplementation with lycium barbarum polysaccharide (LBP) on the developmental competence of vitrified-warmed mouse COCs and the possible mechanisms facilitating this process were investigated. The addition of LBP to the maturation medium improved the maturation rate and enhanced the developmental competence of vitrified-warmed mouse COCs. This may reduce endoplasmic reticulum stress (ERS), preventing cell death and activating both PI3K/AKT and MAPK3/1. In conclusion, cryopreservation lowers the maturation rate and developmental competence of COCs, which can be ameliorated by the application of LBP after warming during maturation.
引用
收藏
页码：7 / 11
页数：5
相关论文
共 50 条
  • [21] Ovine Oocytes Vitrified at Germinal Vesicle Stage as Cytoplast Recipients for Somatic Cell Nuclear Transfer (SCNT)
    Moawad, Adel R.
    Choi, Inchul
    Zhu, Jie
    Campbell, Keith H. S.
    CELLULAR REPROGRAMMING, 2011, 13 (04) : 289 - 296
  • [22] Enhanced Cryoprotective Effect of Melatonin and Resveratrol by Coencapsulation: Improved In Vitro Development of Vitrified-Warmed Mouse Germinal Vesicle Oocytes
    Aghaz, Faranak
    Vaisi-Raygani, Asad
    Khazaei, Mozafar
    Arkan, Elham
    BIOPRESERVATION AND BIOBANKING, 2021, 19 (03) : 184 - 193
  • [23] Position of the nucleus in mouse germinal vesicle–stage oocytes with different chromatin configurations
    K. V. Shishova
    E. A. Lavrentyeva
    A. I. Khamidullina
    O. V. Zatsepina
    Russian Journal of Developmental Biology, 2016, 47 : 313 - 319
  • [24] Effect of cytoplasmic replacement at MII stage on the development of mouse oocytes reconstructed by germinal vesicle transfer.
    Nakata, K
    Aono, F
    Kuwayama, M
    Kato, O
    Nagashima, H
    FERTILITY AND STERILITY, 2003, 80 : S195 - S195
  • [25] L-Carnitine Supplementation During Vitrification of Mouse Oocytes at the Germinal Vesicle Stage Improves Preimplantation Development Following Maturation and Fertilization In Vitro
    Moawad, Adel R.
    Tan, Seang Lin
    Xu, Baozeng
    Chen, Hai Ying
    Taketo, Teruko
    BIOLOGY OF REPRODUCTION, 2013, 88 (04)
  • [26] Ceratonia siliqua(Carob) extract improved in vitro development of vitrified-warmed mouse germinal vesicle oocytes: assessment of possible mechanism
    Faramarzi, Azita
    Aghaz, Farank
    Bakhtiari, Mitra
    Roshankhah, Shiva
    Rashidi, Zahra
    Khazaei, Mozafar
    CELL AND TISSUE BANKING, 2021, 22 (01) : 137 - 144
  • [27] Production of good-quality porcine blastocysts by in vitro fertilization of follicular oocytes vitrified at the germinal vesicle stage
    Somfai, T.
    Noguchi, J.
    Kaneko, H.
    Nakai, M.
    Ozawa, M.
    Kashiwazaki, N.
    Egerszegi, I.
    Ratky, J.
    Nagai, T.
    Kikuchi, K.
    THERIOGENOLOGY, 2010, 73 (02) : 147 - 156
  • [28] Effects of cryoprotectants and cryoprotectant combinations on viability and maturation rates of Camelus dromedarius oocytes vitrified at germinal vesicle stage
    Moawad, Moustafa
    Hussein, Hassan A.
    Abd El-Ghani, Mohamed
    Darwish, Gamal
    Badr, Magdy
    REPRODUCTION IN DOMESTIC ANIMALS, 2019, 54 (01) : 108 - 117
  • [29] Ceratonia siliqua (Carob) extract improved in vitro development of vitrified-warmed mouse germinal vesicle oocytes: assessment of possible mechanism
    Azita Faramarzi
    Farank Aghaz
    Mitra Bakhtiari
    Shiva Roshankhah
    Zahra Rashidi
    Mozafar Khazaei
    Cell and Tissue Banking, 2021, 22 : 137 - 144
  • [30] Position of the Nucleus in Mouse Germinal Vesicle-Stage Oocytes with Different Chromatin Configurations
    Shishova, K. V.
    Lavrentyeva, E. A.
    Khamidullina, A. I.
    Zatsepina, O. V.
    RUSSIAN JOURNAL OF DEVELOPMENTAL BIOLOGY, 2016, 47 (06) : 313 - 319
12345