Label-Free and Continuous-Flow Ferrohydrodynamic Separation of HeLa Cells and Blood Cells in Biocompatible Ferrofluids

被引：83

作者：

Zhao, Wujun ^{[1
]}

Zhu, Taotao ^{[1
]}

Cheng, Rui ^{[2
]}

Liu, Yufei ^{[3
]}

He, Jian ^{[3
]}

Qiu, Hong ^{[4
]}

Wang, Lianchun ^{[4
]}

Nagy, Tamas ^{[5
]}

Querec, Troy D. ^{[6
]}

Unger, Elizabeth R. ^{[6
]}

Mao, Leidong ^{[2
]}

机构：

[1] Univ Georgia, Dept Chem, Athens, GA 30602 USA

[2] Univ Georgia, Coll Engn, 220 Riverbend Rd,Room 166, Athens, GA 30602 USA

[3] Clemson Univ, Dept Phys & Astron, Clemson, SC 29634 USA

[4] Univ Georgia, Complex Carbohydrate Res Ctr, Dept Biochem & Mol Biol, Athens, GA 30602 USA

[5] Univ Georgia, Dept Pathol, Coll Vet Med, Athens, GA 30602 USA

[6] Ctr Dis Control & Prevent, Chron Viral Dis Branch, Div High Consequence Pathogens & Pathol, Natl Ctr Emerging & Zoonot Infect Dis Pathol, Atlanta, GA 30329 USA

来源：

ADVANCED FUNCTIONAL MATERIALS | 2016年 / 26卷 / 22期

基金：

美国国家卫生研究院;

关键词：

INTERLABORATORY COMPARISON PROGRAM; MAGNETIC PARTICLES; MICROFLUIDIC APPLICATIONS; CONVENTIONAL SMEARS; CYTOLOGIC FEATURES; PERFORMED WELL; CHIP; MAGNETOPHORESIS; MANIPULATION; LIQUIDS;

D O I：

10.1002/adfm.201503838

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study, a label-free, low-cost, and fast ferrohydrodynamic cell separation scheme is demonstrated using HeLa cells (an epithelial cell line) and red blood cells. The separation is based on cell size difference, and conducted in a custom-made biocompatible ferrofluid that retains the viability of cells during and after the assay for downstream analysis. The scheme offers moderate-throughput (approximate to 10(6) cells h(-1) for a single channel device) and extremely high recovery rate (>99%) without the use of any label. It is envisioned that this separation scheme will have clinical applications in settings where rapid cell enrichment and removal of contaminating blood will improve efficiency of screening and diagnosis such as cervical cancer screening based on mixed populations in exfoliated samples.

引用

页码：3990 / 3998

页数：9

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