Microtubule-severing protein Fidgetin-like 1 promotes spindle organization during meiosis of mouse oocytes

被引：8

作者：

Shou, Hua-Feng ^{[1
]}

Jin, Zhen ^{[2
]}

Yu, Yan ^{[1
]}

Lai, Yu-Cheng ^{[1
]}

Wu, Qing ^{[1
]}

Gao, Lei-Lei ^{[1
]}

机构：

[1] Zhejiang Prov Peoples Hosp, Hangzhou Med Coll, Affiliated Peoples Hosp, Ctr Reprod Med,Dept Gynecol, Hangzhou 310014, Zhejiang, Peoples R China

[2] Zhejiang Prov Peoples Hosp, Hangzhou Med Coll, Affiliated Peoples Hosp, Ctr Reprod Med,Dept Reprod Endocrinol, Hangzhou 310014, Zhejiang, Peoples R China

来源：

ZYGOTE | 2022年 / 30卷 / 06期

关键词：

Meiosis; Microtubule; Microtubule-severing proteins; Oocyte; Spindle; HEREDITARY SPASTIC PARAPLEGIA; CHROMOSOME SEGREGATION; GAMMA-TUBULIN; AAA-ATPASE; KATANIN; IDENTIFICATION; COMPLEX; POLE; PROGRESSION; INFERTILITY;

D O I：

10.1017/S0967199422000417

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Microtubule-severing proteins (MTSPs) play important roles in mitosis and interphase. However, to the best of our knowledge, no previous studies have evaluated the role of MTSPs in female meiosis in mammals. It was found that FIGNL1, a member of MTSPs, was predominantly expressed in mouse oocytes and distributed at the spindle poles during meiosis in the present study. FIGNL1 was co-localized and interacted with gamma-tubulin, an important component of the microtubule tissue centre (MTOC). Fignl1 knockdown by specific small interfering RNA caused spindle defects characterized by an abnormal length:width ratio and decreased microtubule density, which consequently led to aberrant chromosome arrangement, oocyte maturation and fertilization obstacles. In conclusion, the present results suggested that FIGNL1 may be an essential factor in oocyte maturation by influencing the meiosis process via the formation of spindles.

引用

页码：872 / 881

页数：10