Selection of human antibody fragments by phage display

被引：193
|
作者：
Lee, Carol M. Y. [1 ]
Iorno, Niccolo [1 ]
Sierro, Frederic [1 ]
Christ, Daniel [1 ]
机构：
[1] Garvan Inst Med Res, Sydney, NSW 2010, Australia
来源：
NATURE PROTOCOLS | 2007年 / 2卷 / 11期
关键词：
D O I：
10.1038/nprot.2007.448
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Here, we describe a protocol for the selection of human antibody fragments using repertoires displayed on filamentous bacteriophage. Antigen-specific clones are enriched by binding to immobilized antigen, followed by elution and repropagation of phage. After multiple rounds of binding selection, specific clones are identified by ELISA. This article provides an overview of phage display and antibody technology, as well as detailed protocols for the immobilization of antigen, the selection of repertoires on purified or complex antigens and the identification of binders.
引用
收藏
页码：3001 / 3008
页数：8
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