Effect of knockout of α-carbonic anhydrase 4 gene on photosynthetic characteristics and starch accumulation in leaves of Arabidopsis thaliana

被引：14
|
作者：
Zhurikova, E. M. [1 ]
Ignatova, L. K. [1 ]
Semenova, G. A. [2 ]
Rudenko, N. N. [1 ]
Mudrik, V. A. [1 ]
Ivanov, B. N. [1 ]
机构：
[1] Russian Acad Sci, Inst Basic Biol Problems, Pushchino 142290, Moscow Oblast, Russia
[2] Russian Acad Sci, Inst Theoret & Expt Biophys, Moscow 142290, Moscow Oblast, Russia
来源：
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY | 2015年 / 62卷 / 04期
基金：
俄罗斯基础研究基金会;
关键词：
Arabidopsis thaliana; carbonic anhydrase; photosynthesis; nonphotochemical quenching; starch; ELECTRON-TRANSPORT; FLUORESCENCE; CHLOROPLASTS; VIOLAXANTHIN; INHIBITION; EXPRESSION; ZEAXANTHIN;
D O I：
10.1134/S1021443715040214
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Effects of knockout of the gene encoding alpha-carbonic anhydrase 4 (alpha-CA4) on growth and photosynthesis of Thale cress (Arabidopsis thaliana (L.) Heynh., var. Columbia) were investigated. The shoot weight of mutant plants was found to be higher than in wild-type plants, and the leaves of mutants were enriched in starch content. The electron microscopy study revealed a considerable increase in the number and size of starch grains in chloroplasts of mutant plants. Comparison of wild-type and mutant plant leaves in terms of chlorophyll a fluorescence, coefficient of photochemical fluorescence quenching, effective quantum yield of photosystem II reaction, and nonphotochemical fluorescence quenching under steady-state illumination and saturating CO2 content in the air led to the proposal that alpha-CA4 participates in the development of nonphotochemical energy dissipation by accelerating the supply of protons for activation of violaxanthin deepoxidase and for structural changes in the light-harvesting complexes.
引用
收藏
页码：564 / 569
页数：6
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