Enzymatic synthesis of L-tryptophan from hair acid hydrolysis industries wastewater with tryptophan synthase

被引：13

作者：

Zhao, Genhai ^{[1
]}

Liu, Junzhong ^{[1
]}

Dong, Kai ^{[1
]}

Zhang, Fei ^{[1
]}

Zhang, Hongjuan ^{[1
]}

Liu, Qian ^{[1
]}

Jiao, Qingcai ^{[1
]}

机构：

[1] Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 03期

关键词：

Hair acid hydrolysis wastewater; Enzymatic synthesis; Tryptophan; Tryptophan synthase; ESCHERICHIA-COLI; AMINO-ACIDS; L-SERINE; INHIBITION;

D O I：

10.1016/j.biortech.2010.09.019

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

An effective method for production of L-tryptophan from hair acid hydrolysis wastewater (HEM) containing L-serine was developed by recombinant tryptophan synthase. This study provides us with an alternative HHW utilization strategy. Tryptophan synthase could efficiently convert L-serine contained in HHW to L-tryptophan at pH 8.0, 40 degrees C and Tween-80 of 0.04%. The enzyme also showed high tolerance to ammonium chloride, a component in HHW. In a scale up study, L-serine conversion rate reach 95.1% with a final L-tryptophan concentration of 33.2 g l(-1). (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：3554 / 3557

页数：4

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