Enzymatic synthesis of L-tryptophan from hair acid hydrolysis industries wastewater with tryptophan synthase

被引：13
|
作者：
Zhao, Genhai [1 ]
Liu, Junzhong [1 ]
Dong, Kai [1 ]
Zhang, Fei [1 ]
Zhang, Hongjuan [1 ]
Liu, Qian [1 ]
Jiao, Qingcai [1 ]
机构：
[1] Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Peoples R China
来源：
BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 03期
关键词：
Hair acid hydrolysis wastewater; Enzymatic synthesis; Tryptophan; Tryptophan synthase; ESCHERICHIA-COLI; AMINO-ACIDS; L-SERINE; INHIBITION;
D O I：
10.1016/j.biortech.2010.09.019
中图分类号：
S2 [农业工程];
学科分类号：
0828 ;
摘要：
An effective method for production of L-tryptophan from hair acid hydrolysis wastewater (HEM) containing L-serine was developed by recombinant tryptophan synthase. This study provides us with an alternative HHW utilization strategy. Tryptophan synthase could efficiently convert L-serine contained in HHW to L-tryptophan at pH 8.0, 40 degrees C and Tween-80 of 0.04%. The enzyme also showed high tolerance to ammonium chloride, a component in HHW. In a scale up study, L-serine conversion rate reach 95.1% with a final L-tryptophan concentration of 33.2 g l(-1). (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：3554 / 3557
页数：4
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