Fission yeast Ctf1, a cleavage and polyadenylation factor subunit is required for the maintenance of genomic integrity

被引：0
|
作者：
Sonkar, Amit [1 ,2 ]
Gaurav, Sachin [1 ]
Ahmed, Shakil [1 ]
机构：
[1] Cent Drug Res Inst, CSIR, Mol & Struct Biol Div, Sect 10,Sitapur Rd, Lucknow 226031, Uttar Pradesh, India
[2] North Eastern Hill Univ, Dept Biochem, Shillong, Meghalayn, India
来源：
MOLECULAR GENETICS AND GENOMICS | 2017年 / 292卷 / 05期
关键词：
Ctf1; Rna15; S; pombe; Chromosome integrity; Spindle checkpoint; PRE-MESSENGER-RNA; SPINDLE ASSEMBLY CHECKPOINT; SCHIZOSACCHAROMYCES-POMBE; FACTOR-I; PROTEIN; RECONSTITUTION; SEPARATION; STABILITY; INSIGHTS; REVEALS;
D O I：
10.1007/s00438-017-1329-x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Accurate segregation of chromosome during mitosis requires the coordinated action of several cell cycle checkpoints that monitor replication of the genome and the attachment of sister chromatids to the mitotic spindle apparatus. Here we have characterized the fission yeast Ctf1, an ortholog of S. cerevisiae Rna15 in the maintenance of genomic integrity. The ctf1 is nonessential for the cell survival and its deletion strain exhibit cold sensitivity. The ctf1 deleted cells exhibit genetic interaction with spindle checkpoint protein Mad2 and Bub1. The deletion of ctf1 gene affects the chromosomal attachment to the mitotic spindle leading to the accumulation of Bub1-GFP foci. Ctf1 localizes to the nucleus and physically interacts with Rna14, a cleavage and polyadenylation factor.
引用
收藏
页码：1027 / 1036
页数：10
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