Development of a Graphene-Based Aptamer Sensor for Electrochemical Detection of Serum ECP Levels

被引：2
|
作者：
Wang, Zhitao [1 ]
Yang, Jun [2 ]
Gui, Lin [3 ]
机构：
[1] Wuchang Hosp, Dept Pediat, Wuhan 430063, Hubei, Peoples R China
[2] Xiangaoxin Hosp, Dept Pediat, Xian 710075, Shanxi, Peoples R China
[3] Maternal & Child Hlth Hosp Hubei Prov, Dept Paediat, Wuhan 430070, Hubei, Peoples R China
来源：
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE | 2017年 / 12卷 / 10期
关键词：
Eosinophil cationic protein; Aptamer sensor; Graphene; Electrochemical determination; Children asthmatic attack; GOLD NANOPARTICLES; BIOSENSORS; ASTHMA; INFLAMMATION;
D O I：
10.20964/2017.10.54
中图分类号：
O646 [电化学、电解、磁化学];
学科分类号：
081704 ;
摘要：
The eosinophil cationic protein (ECP) is the biological marker for bronchial eosinophil inflammation, and it can be detected in serological levels to objectively predict the activity and severity of asthma in children. This work reports a facile electrochemical aptasensor without labels for the detection of ECP using a glassy carbon electrode (GCE) modified by Au nanoparticles dotted with a graphene (GNPs/GR) nanocomposite film. The ECP-aptamer interactions were studied using ferricyanide as the electrochemical probe. The terminal GNPs/GR layer displayed an acceptable current for ECP determination. The peak current variation of ferricyanide was linearly related to the ECP concentration (0.01 mu M - 10 mu M), and the limit of detection (LOD) was 4 nM.
引用
收藏
页码：9502 / 9511
页数：10
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