Identification of Novel and Conserved microRNAs in Rehmannia glutinosa L. by Solexa Sequencing

被引：18
|
作者：
Yang, Yanhui [1 ]
Chen, Xinjian [1 ]
Chen, Junying [1 ]
Xu, Haixia [1 ]
Li, Juan [1 ]
Zhang, Zhongyi [1 ]
机构：
[1] Henan Agr Univ, Coll Agron, Zhengzhou 450002, Henan Province, Peoples R China
来源：
PLANT MOLECULAR BIOLOGY REPORTER | 2011年 / 29卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Solexa sequencing; Identification of miRNAs; R; glutinosa; qRT-PCR; Target genes; PLANT MICRORNAS; SMALL RNAS; ARABIDOPSIS-THALIANA; REGULATORY ROLES; PROTEIN; GENES; TRANSCRIPTOME; BIOGENESIS; MUTATION; ACID;
D O I：
10.1007/s11105-011-0293-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the use of a high throughput (Solexa) sequencing platform to obtain the sequences of a large number of microRNAs (miRNAs) from the Chinese medicinal plant Rehmannia glutinosa L. The analysis of > 14,000,000 sequence reads allowed the identification of seven novel miRNAs (comprising seven miRNA families) and conserved 89 miRNAs (comprising 25 miRNA families) in R. glutinosa. The relative abundance of some these miRNAs was checked using quantitive PCR (qRT-PCR) analysis. Some putative target genes of the novel miRNAs were predicted.
引用
收藏
页码：986 / 996
页数：11
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