Hydrogen production by immobilized Synechocystis sp PCC 6803

被引：49
|
作者：
Touloupakis, Eleftherios [1 ]
Rontogiannis, George [2 ]
Silva Benavides, Ana Margarita [3 ,4 ]
Cicchi, Bernardo [1 ]
Ghanotakis, Demetrios F. [2 ]
Torzillo, Giuseppe [1 ]
机构：
[1] CNR, Ist Studio Ecosistemi, Via Madonna Piano 10, I-50019 Sesto Fiorentino, Italy
[2] Univ Crete, Dept Chem, Voutes Heraklion 71003, Greece
[3] Univ Costa Rica, Escuela Biol, San Jose 2060, Costa Rica
[4] Univ Costa Rica, Ctr Invest Ciencias Mar & Limnol CIMAR, San Jose 2060, Costa Rica
来源：
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY | 2016年 / 41卷 / 34期
关键词：
Synechocystis sp PCC 6803; Alginate; Immobilization; Biohydrogen; CHLAMYDOMONAS-REINHARDTII; GLOEOCAPSA-ALPICOLA; CYANOBACTERIAL HYDROGENASES; BIDIRECTIONAL HYDROGENASE; H-2; PRODUCTION; PHOTOPRODUCTION; GROWTH; CULTURES; REMOVAL; MEDIA;
D O I：
10.1016/j.ijhydene.2016.07.075
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The ability of the immobilized Synechocystis sp. PCC 6803 cells to produce hydrogen via an indirect light driven process was investigated. Cells were successfully immobilized in a calcium alginate gel matrix. Beads were prepared by using 3% (w/v) alginate and 2% (w/v) calcium chloride, and were stable showing minimal cell leakage. Beads had a mean diameter of 5.06 +/- 0.18 mm, a volume of 67.8 +/- 2.4 mu L and a mean cell dry weight of 46.3 +/- 0.8 mu g. Immobilized cells retained their viability, as shown by measurement of the F-v/F-m ratio, for at least 30 days. The maximum rate of hydrogen production reached 5.73 +/- 0.69 mL H-2 mg cells(-1) (e.g., 40.6 +/- 4.9 mu mol H-2 mg chl(-1) h(-1)). (C) 2016 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：15181 / 15186
页数：6
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