Fluorescence Microscopy Visualization of Contacts Between Objects

被引：55

作者：

Suhina, Tomislav ^{[1
,2
]}

Weber, Bart ^{[2
]}

Carpentier, Chantal E. ^{[1
,2
]}

Lorincz, Kinga ^{[2
]}

Schall, Peter ^{[2
]}

Bonn, Daniel ^{[2
]}

Brouwer, Albert M. ^{[1
]}

机构：

[1] Univ Amsterdam, Vant Hoff Inst Mol Sci, NL-1090 GD Amsterdam, Netherlands

[2] Univ Amsterdam, Inst Phys, NL-1090 GL Amsterdam, Netherlands

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2015年 / 54卷 / 12期

关键词：

fluorescent probes; interfaces; mechanical properties; surface chemistry; DCDHF FLUOROPHORES; VISCOSITY; TEMPERATURE; FRICTION;

D O I：

10.1002/anie.201410240

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The area of contact between two objects was detected by using the strong enhancement of the fluorescence of rigidochromic probe molecules attached to one of the surfaces. Confinement of the molecules suppresses nonradiative decay and turns on the fluorescence. The approach is demonstrated by imaging of the contact area of a plastic sphere in contact with a flat glass surface. Our results agree excellently with the prediction of Hertz's classical theory based on elastic deformation.

引用

页码：3688 / 3691

页数：4

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