ISOLATION AND MOLECULAR DETECTION OF ARTHROBACTER SPECIES GROWN ON THE SURFACE OF DATE PALM TISSUE CULTURE

被引：0
|
作者：
Abdulrazzaq, A. [1 ]
Khierallah, H. S. [1 ]
Al-Rubaye, S. M. H. [1 ]
Bader, S. M. [1 ]
机构：
[1] Univ Baghdad, Coll Vet Med, Dept Microbiol, Baghdad, Iraq
来源：
IRAQI JOURNAL OF AGRICULTURAL SCIENCES | 2019年 / 50卷 / 05期
关键词：
shoot tips; Glutamicibacter arilaitensis; polymerase chain reaction; hormons; antibiotic sensitivity; SP NOV; SMEAR; ACTINOMYCETE; BACTERIA; SOFT; SOIL;
D O I：
暂无
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
This study was aimed to found out the type of bacterial species grown on the surface of date palm tissue culture media. Shoot tips of date palm (Barhi cv.) were cultured on MS media supplemented with various combinations of hormones. During growth an infection was appeared in all cultures with gray colour. Primarily identification, proved a bacterial infection due to its colour and appearance. The genus Arthrobacter was isolated Antibiotic sensitivity test showed that the isolate was sensitive to ciprofloxacin,Trimethoprim, and Amikacin and resistant to Clarithromycin,Ceftriaxone and Gentamycin. Sequence analysis of 16 rRNA indicated a new isolates were closely related to the Glutamicibacter arilaitensis strain ebst40 and Arthrobacter arilaitensis strain L11 with the highest sequence similarity (100%) .
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页码：1425 / 1431
页数：7
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