Thai Acanthamoeba isolate (T4) induced apoptotic death in neuroblastoma cells via the Bax-mediated pathway

被引：15
|
作者：
Dharmkrong-at Chusattayanond, Araya [1 ]
Boonsilp, Siriphan [1 ]
Kasisit, Jitra [1 ]
Boonmee, Atsadang [1 ]
Warit, Saradee [2 ]
机构：
[1] Mahidol Univ, Fac Sci, Dept Microbiol, Bangkok 10400, Thailand
[2] Natl Ctr Genet Engn & Biotechnol NSTDA, TB Res Lab, Pathum Thani 10120, Thailand
来源：
PARASITOLOGY INTERNATIONAL | 2010年 / 59卷 / 04期
关键词：
Acanthamoeba; T4; genotype; Apoptosis; Caspase; Bax; Bcl; 2; KERATITIS; IDENTIFICATION; SPECIMENS; INCREASE; FAMILY; ADP;
D O I：
10.1016/j.parint.2010.06.007
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
A Thai Acanthamoeba isolate named AS recovered from a corneal scraping of a keratitis patient was genotypically determined as T4 AS trophozoites were used for studying Acanthamoeba-induced apoptosis in mouse neuroblastoma NA cells during in vitro co-cultivation The Acanthamoeba-ex posed NA cells showed signs of apoptosis including cell shrinkage nuclear condensation and DNA fragmentation The effect was confirmed by DNA laddering electrophoresis Involvement of caspase enzymes and mitochondrial pro- and anti apoptotic proteins (Bax and Bcl 2) in AS-induced apoptosis was determined The use of Z-VAD-FMK a pan-caspase inhibitor significantly reduced the apoptotic effect while Bax/Bcl-2 ratio analysis showed a significant increase in the expression of apoptotic proteins in AS-exposed NA cells These results strongly indicated that apoptosis induced by AS trophozoites is caspase-dependent and is mediated by over expression of pro-apoptotic proteins in the mitochondrial pathway This is the first report on the role of Bax in mediating apoptosis induced by Acanthamoeba (C) 2010 Elsevier Ireland Ltd All rights reserved
引用
收藏
页码：512 / 516
页数：5
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