A functional study on saposin B and C using experimentally validated models

To speed up the structure-based analysis of protein function, we have developed the concept of Experimentally Validated 3D Models (EVAMs), i.e., structural models that can be generated relatively fast by a combination of bioinformatics and experiments. Here, we test the suitability of EVAMs for functional studies by applying the concept to two small proteins, saposin B and saposin C. We find that EVAMs are well able to explain some of the functional properties of these proteins, such as their pH-dependent association with lipid bilayers, that are known from independent biophysical experiments.