A method for high-throughput image-based antifungal screening

被引：3
|
作者：
Sabburg, Rosalie [1 ]
Gregson, Aphrika [1 ,2 ,3 ]
Urquhart, Andrew S. [1 ,4 ]
Aitken, Elizabeth A. B. [2 ]
Smith, Linda [5 ]
Thatcher, Louise F. [6 ]
Gardiner, Donald M. [1 ,2 ]
机构：
[1] CSIRO, St Lucia, Qld, Australia
[2] Univ Queensland, St Lucia, Qld, Australia
[3] New South Wales Dept Primary Ind, Narrabri, NSW, Australia
[4] Macquarie Univ, Appl Biosci, Macquarie Pk, NSW, Australia
[5] Dept Agr & Fisheries, Dutton Pk, Qld, Australia
[6] CSIRO, Black Mt, ACT, Australia
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2021年 / 190卷
关键词：
Antifungal; Verticillium; Aspergillus; Fusarium; Rapid; Susceptibility; SELECTION;
D O I：
10.1016/j.mimet.2021.106342
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Robust antifungal screening is technically challenging particularly for filamentous fungi. We present a method for undertaking antifungal screening assays that builds upon existing broth dilution protocols and incorporates time resolved image-based assessment of fungal growth. We show that the method performs with different fungi, particularly those for which spores can be used as inoculum, and with different compound classes, can accurately assess susceptibility or otherwise in only few hours and can even account for differences in inherent growth properties of strains.
引用
收藏
页数：7
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