Highly Selective Fluorescent Probe for Vicinal-Dithiol-Containing Proteins and In Situ Imaging in Living Cells

被引：71

作者：

Huang, Chusen ^{[1
]}

Yin, Qin ^{[1
]}

Zhu, Weiping ^{[1
]}

Yang, Yi ^{[1
]}

Wang, Xin ^{[1
]}

Qian, Xuhong ^{[1
]}

Xu, Yufang ^{[1
]}

机构：

[1] E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab Chem Biol, Shanghai, Peoples R China

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 33期

基金：

国家高技术研究发展计划(863计划); 中国国家自然科学基金;

关键词：

fluorescent probes; in situ imaging; protein labeling; redox proteomics; vicinal dithiols; MAMMALIAN-CELLS; FREE-RADICALS; THIOL-GROUPS; ACID; IDENTIFICATION; OXIDATION; STRESS; QUANTIFICATION; DISULFIDES; FLUOROGENS;

D O I：

10.1002/anie.201101317

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

It pays to be direct: In a rapid and specific approach to the detection of vicinal-dithiol-containing proteins (VDPs), the use of a fluorescent probe (see picture) enabled the direct readout of fluorescence. This approach based on fluorescence polarization, electrophoresis, and the direct imaging of VDPs permits the noninvasive study of VDPs both in vitro and in living cells and offers insight into their potential roles in cell function. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：7551 / 7556

页数：6

共 50 条

[1] Versatile Probes for the Selective Detection of Vicinal-Dithiol-Containing Proteins: Design, Synthesis, and Application in Living Cells
Huang, Chusen
Yin, Qin
Meng, Jiangjiang
Zhu, Weiping
Yang, Yi
Qian, Xuhong
Xu, Yufang
[J]. CHEMISTRY-A EUROPEAN JOURNAL, 2013, 19 (24) : 7739 - 7747
[2] Development of a red fluorescent light-up probe for highly selective and sensitive detection of vicinal dithiol-containing proteins in living cells
Wang, Yuanyuan
Yang, Xiao-Feng
Zhong, Yaogang
Gong, Xueyun
Li, Zheng
Li, Hua
[J]. CHEMICAL SCIENCE, 2016, 7 (01) : 518 - 524
[3] In vivo precision imaging of vicinal-dithiol-containing proteins by a FRET molecular probe sensitive to protein environment
Gong, Haocheng
Zhang, Yanhui
Gao, Ying
Tian, Xinrong
Wu, Pengzhao
Wei, Xiaoyu
Guo, Yuan
[J]. CHINESE CHEMICAL LETTERS, 2023, 34 (12)
[4] In vivo precision imaging of vicinal-dithiol-containing proteins by a FRET molecular probe sensitive to protein environment
Haocheng Gong
Yanhui Zhang
Ying Gao
Xinrong Tian
Pengzhao Wu
Xiaoyu Wei
Yuan Guo
[J]. Chinese Chemical Letters, 2023, 34 (12) : 478 - 481
[5] Far-Red Fluorescent Probe for Imaging of Vicinal Dithiol-Containing Proteins in Living Cells Based on a pKa Shift Mechanism
Zhang, Shengrui
Chen, Guojun
Wang, Yuanyuan
Wang, Qin
Zhong, Yaogang
Yang, Xiao-Feng
Li, Zheng
Li, Hua
[J]. ANALYTICAL CHEMISTRY, 2018, 90 (04) : 2946 - 2953
[6] A β-allyl carbamate fluorescent probe for vicinal dithiol proteins
Zhao, Lanning
Bai, Feifei
Chen, Fan
Guo, Menghuan
Gan, Lu
Zhang, Hong
Fang, Jianguo
[J]. CHEMICAL COMMUNICATIONS, 2020, 56 (19) : 2857 - 2860
[7] Mitochondrion-Targeting, Environment-Sensitive Red Fluorescent Probe for Highly Sensitive Detection and Imaging of Vicinal Dithiol-Containing Proteins
Liu, Feng
Liu, Hai-Juan
Liu, Xian-Jun
Chen, Wen
Wang, Fenglin
Yu, Ru-Qin
Jiang, Jian-Hui
[J]. ANALYTICAL CHEMISTRY, 2017, 89 (21) : 11203 - 11207
[8] A highly selective fluorescent probe for the detection and imaging of peroxynitrite in living cells
Yang, Dan
Wang, Hua-Li
Sun, Zhen-Ning
Chung, Nga-Wai
Shen, Jian-Gang
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (18) : 6004 - 6005
[9] A Highly Selective Fluorescent Probe for Imaging Hydrogen Sulfide in Living HeLa Cells
Zhang, Wenshen
Xun, Qining
Xing, Wenfang
Xu, Feng
Liu, Xia
[J]. JOURNAL OF FLUORESCENCE, 2023, 33 (04) : 1603 - 1608
[10] A Highly Selective Fluorescent Probe for Imaging Hydrogen Sulfide in Living HeLa Cells
Wenshen Zhang
Qining Xun
Wenfang Xing
Feng Xu
Xia Liu
[J]. Journal of Fluorescence, 2023, 33 : 1603 - 1608

← 1 2 3 4 5 →