Whole-column-imaging detection for capillary isoelectric focusing and capillary electrophoresis

被引:0
|
作者
Wu, XZ
Wu, JQ
Pawliszyn, J
机构
[1] Univ Waterloo, Dept Chem, Waterloo, ON N2L 3G1, Canada
[2] Convergent Biosci Ltd, Toronto, ON 8MZ 2L8, Canada
关键词
D O I
暂无
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this article, the authors review recent developments in the research of whole-column-imaging detection for capillary electrophoresis (E). Whole-column-imaging detection was developed for capillary isoelectric focusing, for which it proved to be an ideal detector. Several whole-column-imaging detectors - including refractive index gradient imaging, UV-absorption imaging, and fluorescence imaging detectors - have been studied. The capillary isoelectric focusing UV-absorption imaging technique even has been commercialized. The development of whole-column-imaging detection itself facilitates CE studies in many directions such as in electophoretic dynamics within narrow channels, new separation modes, and two-dimensional separations. Whole-column-imaging detection also finds application in capillary zone electrophoresis.
引用
收藏
页码:526 / +
页数:12
相关论文
共 50 条
  • [31] Isoelectric focusing of monoclonal antibodies by capillary electrophoresis
    Kundu, S
    Fenters, C
    JOURNAL OF CAPILLARY ELECTROPHORESIS, 1995, 2 (06) : 273 - 277
  • [32] Fraction collection in micropreparative capillary zone electrophoresis and capillary isoelectric focusing
    Minarik, M
    Foret, F
    Karger, BL
    ELECTROPHORESIS, 2000, 21 (01) : 247 - 254
  • [33] Rapid and high-resolution glycoform profiling of recombinant human erythropoietin by capillary isoelectric focusing with whole column imaging detection
    Dou, Peng
    Liu, Zhen
    He, Jiangang
    Xu, Jing-Juan
    Chen, Hong-Yuan
    JOURNAL OF CHROMATOGRAPHY A, 2008, 1190 (1-2) : 372 - 376
  • [34] Determination of the isoelectric point of shortened glucagon-like peptide-1 by capillary isoelectric focusing with whole column imaging
    Fang, Junjian
    Liu, Jiexin
    Li, Haijing
    Cheng, Jianhua
    Wu, Shengming
    Dong, Fangting
    INSTRUMENTATION SCIENCE & TECHNOLOGY, 2018, 46 (05) : 545 - 554
  • [35] Isoelectric Focusing Sample Injection for Capillary Zone Electrophoresis in a Fused Silica Capillary
    Luo-Hong Zhang
    Cheng-Jie Zhang
    Xing-Zheng Wu
    Analytical Sciences, 2006, 22 : 1039 - 1041
  • [36] Capillary isoelectric focusing of erythropoietin glycoforms and its comparison with flat-bed isoelectric focusing and capillary zone electrophoresis
    Cifuentes, A
    Moreno-Arribas, MV
    de Frutos, M
    Díez-Masa, JC
    JOURNAL OF CHROMATOGRAPHY A, 1999, 830 (02) : 453 - 463
  • [37] ISOELECTRIC-FOCUSING BY FREE SOLUTION CAPILLARY ELECTROPHORESIS
    CHEN, SM
    WIKTOROWICZ, JE
    ANALYTICAL BIOCHEMISTRY, 1992, 206 (01) : 84 - 90
  • [38] Capillary electrophoresis and isoelectric focusing in peptide and protein analysis
    Righetti, Pier Giorgio
    Sebastiano, Roberto
    Citterio, Attilio
    PROTEOMICS, 2013, 13 (02) : 325 - 340
  • [39] Isoelectric focusing sample injection for capillary electrophoresis of proteins
    Wu, XZ
    Zhang, LH
    Onoda, K
    ELECTROPHORESIS, 2005, 26 (03) : 563 - 570
  • [40] Isoelectric focusing sample injection for capillary zone electrophoresis in a fused silica capillary
    Zhang, Luo-Hong
    Zhang, Cheng-Jie
    Wu, Xing-Zheng
    ANALYTICAL SCIENCES, 2006, 22 (07) : 1039 - 1041