Whole-column-imaging detection for capillary isoelectric focusing and capillary electrophoresis

被引:0
|
作者
Wu, XZ
Wu, JQ
Pawliszyn, J
机构
[1] Univ Waterloo, Dept Chem, Waterloo, ON N2L 3G1, Canada
[2] Convergent Biosci Ltd, Toronto, ON 8MZ 2L8, Canada
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中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
In this article, the authors review recent developments in the research of whole-column-imaging detection for capillary electrophoresis (E). Whole-column-imaging detection was developed for capillary isoelectric focusing, for which it proved to be an ideal detector. Several whole-column-imaging detectors - including refractive index gradient imaging, UV-absorption imaging, and fluorescence imaging detectors - have been studied. The capillary isoelectric focusing UV-absorption imaging technique even has been commercialized. The development of whole-column-imaging detection itself facilitates CE studies in many directions such as in electophoretic dynamics within narrow channels, new separation modes, and two-dimensional separations. Whole-column-imaging detection also finds application in capillary zone electrophoresis.
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页码:526 / +
页数:12
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