Two-Photon Excitation/Red Emission, Ratiometric Fluorescent Nanoprobe for Intracellular pH Imaging

被引：38

作者：

Wang, Ningning ^{[1
]}

Yu, Xinyan ^{[1
]}

Deng, Ting ^{[1
]}

Zhang, Ke ^{[1
]}

Yang, Ronghua ^{[2
]}

Li, Jishan ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, Inst Chem Biol & Nanomed, State Key Lab Chemo Biosensing & Chemometr, Changsha 410082, Hunan, Peoples R China

[2] Changsha Univ Sci & Technol, Sch Chem & Biol Engn, Changsha 410114, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2020年 / 92卷 / 01期

关键词：

TRACKING DYNAMIC CHANGES; LIVING CELLS; ACIDIC PH; PROBE; NANOPARTICLES; VISUALIZATION; TRAFFICKING; APOPTOSIS; RESPONSES; POLYMER;

D O I：

10.1021/acs.analchem.9b04782

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Herein, we describe a novel two-photon excitation/red emission-based ratiometric pH nanosensor consisting of a pH-sensitive two-photon dye and Tm3+-doped upconversion nanoparticles (UCNP). The fluorescence emission ratio between the dye (610 nm) and UCNPs (810 nm) (I-610/I-810) provides a linear indicator of pH values in the range from pH 4.0 to 6.5 with high sensitivity. These nanoprobes selectively accumulate in the lysosomes of cells, making them suitable for lysosomal pH tracking. This pH nanoprobe has been successfully applied in visualizing chemically stimulated changes of intracellular pH in living cells and tissues.

引用

页码：583 / 587

页数：5

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