Structural and functional studies of hemoglobin Moabit (alpha 86(F7)) Leu->Arg

被引：0
|
作者：
Gulbis, B
Tshilolo, L
Wajcman, H
Riou, J
Galacteros, F
Prome, D
Kister, J
Papassotiriou, I
VanLaethem, Y
Vertongen, F
机构：
[1] HOP UNIV ST PIERRE, B-1000 BRUSSELS, BELGIUM
[2] HOP HENRI MONDOR, INSERM U91, UNITE RECH GENET MOL & HEMATOL, F-94010 CRETEIL, FRANCE
[3] CNRS, PHARMACOL & TOXICOL FONDAMENTALES LAB, F-31077 TOULOUSE, FRANCE
[4] HOP BICETRE, INSERM U299, LE KREMLIN BICETRE, FRANCE
来源：
HEMATOLOGY AND CELL THERAPY | 1997年 / 39卷 / 03期
关键词：
hemoglobin Moabit; electrophoresis; globin chains; chromatography;
D O I：
10.1007/s00282-997-0137-x
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
An abnormal hemoglobin fraction was detected on high performance liquid chromatography profile performed for the measurement of glycated hemoglobin in a 55-year-old caucasian patient. The structural and functional studies were performed by standard techniques. Separation of hemoglobins by alkaline electrophoresis and by IEF revealed a slightly more rapid fraction than does Hb S. By acid electrophoresis, no abnormal Hb fraction could be observed. Separation of globin chains by electrophoresis demonstrated an alpha-chain variant and by chromatography, a fraction which eluted between beta and gamma globin chains. Tryptic digests and amino acid analysis have demonstrated a previously described substitution of Leu --> Arg alpha 86(F7).
引用
收藏
页码：137 / 138
页数：2
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