In silico genome-wide screening for TnrA-regulated genes of Bacillus clausii

被引:0
|
作者
Farazmand, Abbas [1 ,2 ]
Yakhchali, Bagher [1 ]
Shariati, Parvin [1 ]
Minuchehr, Zarrin [1 ]
Ofoghi, Hamideh [2 ]
机构
[1] NIGEB, Dept Ind & Environm Biotechnol, Tehran, Iran
[2] IROST, Dept Biotechnol, Tehran, Iran
关键词
Gene regulation; TnrA; Bacillus clausii; nitrogen metabolism; SUBTILIS; EXPRESSION;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bacillus clausii TnrA transcription factor is required for global nitrogen regulation. In order to obtain an overview of gene regulation by TnrA in B. clausii KSM-K16, the entire genome of B. clausii was screened for the consensus sequence, 5'-TGTNAN7TNACA-3' known as the TnrA box, and 13 transcription units were found containing a putative TnrA box. The TnrA targets identified in this study were tnrA, glnA, nrgA, nasFDEB, puc genes, licT, the two operons of the oligopeptide ABC transporter, lytR, transcriptional regulator of the Lrp/AsnC family, sodium-dependent transporter of SNF family, hyu genes and a biochemically uncharacterized protein.
引用
收藏
页码:61 / 65
页数:5
相关论文
共 50 条
  • [31] Pooled Genome-Wide shRNA Screening
    Holemon, Heather
    Rusakova, Victoria
    GENETIC ENGINEERING & BIOTECHNOLOGY NEWS, 2009, 29 (20): : 40 - 41
  • [32] A CRISPR method for genome-wide screening
    Brooke LaFlamme
    Nature Genetics, 2014, 46 (2) : 99 - 99
  • [33] Genome-wide screening for genes involved in the epigenetic basis of fragile X syndrome
    Vershkov, Dan
    Yilmaz, Atilgan
    Yanuka, Ofra
    Nielsen, Anders Lade
    Benvenisty, Nissim
    STEM CELL REPORTS, 2022, 17 (05): : 1048 - 1058
  • [34] Identification of new keratinocyte differentiation candidates genes by a genome-wide screening approach
    Freiberg, R
    Barry, C
    Brown, P
    Khavari, P
    JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1999, 112 (04) : 624 - 624
  • [35] Genome-wide functional screening of drug-resistance genes in Plasmodium falciparum
    Shiroh Iwanaga
    Rie Kubota
    Tsubasa Nishi
    Sumalee Kamchonwongpaisan
    Somdet Srichairatanakool
    Naoaki Shinzawa
    Din Syafruddin
    Masao Yuda
    Chairat Uthaipibull
    Nature Communications, 13
  • [36] Genome-wide identification of genes regulated in trans by transposable element small interfering RNAs
    McCue, Andrea D.
    Nuthikattu, Saivageethi
    Slotkin, R. Keith
    RNA BIOLOGY, 2013, 10 (08) : 1379 - 1395
  • [37] Genome-Wide RNAi Screening Identifies Genes Inhibiting the Migration of Glioblastoma Cells
    Yang, Jian
    Fan, Jing
    Li, Ying
    Li, Fuhai
    Chen, Peikai
    Fan, Yubo
    Xia, Xiaofeng
    Wong, Stephen T.
    PLOS ONE, 2013, 8 (04):
  • [38] Genome-wide screening of yeast metal homeostasis genes involved in mitochondrial functions
    Juexuan Wang
    Xiaoxi Wang
    Ying Fang
    Bing Zhou
    Molecular Genetics and Genomics, 2007, 277 : 673 - 683
  • [39] Genome-Wide Screening for Genes Associated with Valproic Acid Sensitivity in Fission Yeast
    Zhang, Lili
    Ma, Ning
    Liu, Qingbin
    Ma, Yan
    PLOS ONE, 2013, 8 (07):
  • [40] A Genome-Wide Screening in Saccharomyces cerevisiae for Suppressor Genes of MTM1
    Wang Juan
    Zhang Min-Jie
    Zeng Ya-Xue
    Cai Ying
    Zhou Bing
    PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2010, 37 (01) : 42 - 48