Glycoenzyme Tool Development: Principles, Screening Methods, and Recent Advances†

被引：3

作者：

Cui, Tongxiao ^{[1
]}

Man, Yi ^{[1
]}

Wang, Feifei ^{[1
]}

Bi, Shuyang ^{[2
]}

Lin, Liang ^{[2
]}

Xie, Ran ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Coordinat Chem, Chem & Biomed Innovat Ctr ChemBIC, Nanjing 210023, Jiangsu, Peoples R China

[2] Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China

来源：

CHINESE JOURNAL OF CHEMISTRY | 2022年 / 40卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Carbohydrates; Rational design; Directed evolution; High-throughput screening; Glycan detection method; PASTEURELLA-MULTOCIDA SIALYLTRANSFERASE; DIRECTED EVOLUTION; N-ACETYLGLUCOSAMINE; CRYSTAL-STRUCTURES; ESCHERICHIA-COLI; CHEMOENZYMATIC APPROACH; SATURATION MUTAGENESIS; SUBSTRATE-SPECIFICITY; CATALYTIC DOMAIN; BINDING SITES;

D O I：

10.1002/cjoc.202100770

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Comprehensive Summary To elucidate the relationship between carbohydrate structure and its biological function, glycoenzyme tools are widely applied in a variety of physiological and pathological scenarios. Intricate adjustment via glycoenzyme engineering improved the catalytic efficiency, extended the repertoire of accessible catalytic reactions, and tailored novel substrate specificities. In this review, we introduce the principles of glycoenzyme engineering and summarize the recent advances in the rational design and directed evolution of these enzymes, with an emphasis on screening methods in directed evolution. We also excerpt novel detection methods in glycobiology that can be adapted for the development of next-generation glycoenzyme tools.

引用

页码：746 / 758

页数：13

共 50 条

[21] RECENT ADVANCES IN METEOROLOGICAL METHODS
JOHNSON, N
NATURE, 1946, 157 (3983) : 247 - 250
[22] RECENT ADVANCES IN MICROBIOLOGICAL METHODS
不详
NATURE, 1953, 171 (4351) : 507 - 509
[23] Recent Advances in Laboratory Methods
Stovall, W. D.
AMERICAN JOURNAL OF PUBLIC HEALTH AND THE NATIONS HEALTH, 1937, 27 (01): : 50 - 55
[24] RECENT ADVANCES IN SEROTONIN METHODS
AZMITIA, EC
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1982, 30 (08) : 739 - 743
[25] Recent Advances in Neuroimaging Methods
Monchi, Oury
Benali, Habib
Doyon, Julien
Strafella, Antonio P.
INTERNATIONAL JOURNAL OF BIOMEDICAL IMAGING, 2008, 2008 (01)
[26] Recent Advances in the Development of Methods for the Preparation of Functionalized Azulenes for Electrochromic Applications
Ito, Shunji
Shoji, Taku
Morita, Noboru
SYNLETT, 2011, (16) : 2279 - 2298
[27] Recent advances in methods, policies and technologies at sustainable energy systems development
Guzovic, Zvonimir
Duic, Neven
Piacentino, Antonio
Markovska, Natasa
Mathiesen, Brian Vad
Lund, Henrik
ENERGY, 2022, 245
[28] Recent advances in cutting tool materials
Söderberg, S
ADVANCES IN HARD MATERIALS PRODUCTION, 1996, : 19 - 27
[29] Recent advances in virtual ligand screening
Blake, JF
Laird, ER
ANNUAL REPORTS IN MEDICINAL CHEMISTRY, VOL 38, 2003, 38 : 305 - 314
[30] RECENT ADVANCES IN SCREENING THE EMOTIONALLY MALADJUSTED
Zubin, Joseph
JOURNAL OF CLINICAL PSYCHOLOGY, 1948, 4 (01) : 56 - 63

← 1 2 3 4 5 →