Glycoenzyme Tool Development: Principles, Screening Methods, and Recent Advances†

被引：3

作者：

Cui, Tongxiao ^{[1
]}

Man, Yi ^{[1
]}

Wang, Feifei ^{[1
]}

Bi, Shuyang ^{[2
]}

Lin, Liang ^{[2
]}

Xie, Ran ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Coordinat Chem, Chem & Biomed Innovat Ctr ChemBIC, Nanjing 210023, Jiangsu, Peoples R China

[2] Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China

来源：

CHINESE JOURNAL OF CHEMISTRY | 2022年 / 40卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Carbohydrates; Rational design; Directed evolution; High-throughput screening; Glycan detection method; PASTEURELLA-MULTOCIDA SIALYLTRANSFERASE; DIRECTED EVOLUTION; N-ACETYLGLUCOSAMINE; CRYSTAL-STRUCTURES; ESCHERICHIA-COLI; CHEMOENZYMATIC APPROACH; SATURATION MUTAGENESIS; SUBSTRATE-SPECIFICITY; CATALYTIC DOMAIN; BINDING SITES;

D O I：

10.1002/cjoc.202100770

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Comprehensive Summary To elucidate the relationship between carbohydrate structure and its biological function, glycoenzyme tools are widely applied in a variety of physiological and pathological scenarios. Intricate adjustment via glycoenzyme engineering improved the catalytic efficiency, extended the repertoire of accessible catalytic reactions, and tailored novel substrate specificities. In this review, we introduce the principles of glycoenzyme engineering and summarize the recent advances in the rational design and directed evolution of these enzymes, with an emphasis on screening methods in directed evolution. We also excerpt novel detection methods in glycobiology that can be adapted for the development of next-generation glycoenzyme tools.

引用

页码：746 / 758

页数：13

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