Objective(s): Artemisia absinthium is an aromatic, perennial small shrub that shows multiple medical benefits, including anticancerous, neuroprotective, antifungal, hepatoprotective, antidepressant and antioxidant properties. One of the effective approaches to treat Alzheimer's disease is targeting amyloid aggregation by antiamyloid drugs. In the current research study, an excellent grouping of niosomal, lipid nano-carriers drugs containing artemisia absinthium is advanced and characterized to inhibit amyloid aggregation. Materials and Methods: Niosomal vesicles were made employing phosphatidylcholine, span 60, cholesterol and DSPE-PEG2000 by the thin-film method. Then artemisia absinthium was loaded into the niosomes. Their physico-chemical attributes were analyzed utilizing Zeta-Sizer, FTIR, and SEM, and the amount of drug release was measured at 37 degrees C. Finally, the inhibitory effect of artemisia absinthium that loaded niosomal vesicles on the aggregation of amyloid-beta peptides was investigated using Thioflavin T fluorescence measurements and atomic force microscopy. Results: Niosomes containing artemisia absinthium have a size of 174 +/- 2.56nm, the encapsulation efficiency of 66.73%, zeta potential of -26.5 +/- 1/42 mV and polydispersity index (PDI) of 0.373 +/- 0/02. The release of the drug is controlled in this nano-carrier and FTIR and SEM investigations showed that the drug and nano-carrier did not interact and their particles had a spherical structure. In the end, the inhibitory effect of artemisia absinthium that loaded niosomal vesicles on the aggregation of amyloid-beta peptides was examined and confirmed through Thioflavin T fluorescence measurements and atomic force microscopy. Conclusion: Meanwhile, the findings of the current study, confirm the appropriate physicochemical features of the system, a slow-release system, show that this nano-carrier inhibits amyloid aggregation, thus, the nano-niosomes containing essential oil from artemisia absinthium has the capability to preclude amyloid development.
