A new set of primers for COI amplification from purpleback flying squid (Sthenoteuthis oualaniensis)

被引：6
|
作者：
Xu, Lei [1 ]
Huang, Qi [2 ]
Xu, Shaolin [2 ]
Wang, Xuehui [1 ]
Zhang, Peng [1 ]
Xu, Liang [3 ]
Du, Feiyan [1 ]
机构：
[1] Chinese Acad Fishery Sci, Guangdong Prov Key Lab Fishery Ecol & Environm, Key Lab South China Sea Fishery Resources Dev & U, Minist Agr,South China Sea Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China
[2] Jinan Univ, Dept Ecol, Guangzhou, Guangdong, Peoples R China
[3] Shantou Univ, Dept Biol, Shantou, Peoples R China
来源：
MITOCHONDRIAL DNA PART B-RESOURCES | 2017年 / 2卷 / 02期
关键词：
COI; new primer set; Sthenoteuthis oualaniensis; DNA barcodes; MOLLUSCA CEPHALOPODA; DNA;
D O I：
10.1080/23802359.2017.1357439
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Despite the contribution of DNA barcoding towards understanding the biodiversity and distribution of species, the success of the mitochondrial cytochrome c oxidase subunit I gene (COI) amplification has been quite variable when it comes to Cephalopoda. Some species in this class such as Sthenoteuthis oualaniensis seem to be more difficult to amplify COI than others due to failed amplifications with universal primer and lack of specific set of primers. In this study, we developed new Sthenoteuthis - specific primer set, which significantly increased average amplification success. The new primer set will aid the recovery of barcodes from this difficult group and facilitate further studies in phylogeny and cryptic diversity of Sthenoteuthis oualaniensis.
引用
收藏
页码：439 / 443
页数：5
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