Detection of Brucella spp. and Leptospira spp. by multiplex polymerase chain reaction (PCR) from aborted bovine, ovine and caprine fetuses in Iran

被引：13
|
作者：
Moshkelani, Saadat [1 ]
Javaheri-Koupaei, Mohamad [1 ]
Rabiee, Sadegh [2 ]
Moazeni, Mostafa [1 ]
机构：
[1] Islamic Azad Univ, Shahrekord Branch, Shahrekord, Iran
[2] Islamic Azad Univ, Shahrekord Branch, Fac Vet Med, Shahrekord, Iran
来源：
AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH | 2011年 / 5卷 / 26期
关键词：
Multiplex polymerase chain reaction (PCR); Brucella spp; Leptospira spp; abortion; SHEEP; DIAGNOSIS; DNA;
D O I：
10.5897/AJMR11.760
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Brucellosis and leptosrirosis are important causes of livestock abortion, that can be serologically diagnosed. Generally, direct methods as bacteriological isolation are employed, but they are dangerous, taking up much time and not enough reliable. The polymerase chain reaction (PCR) has been reported to be successful for simultaneous detection of microorganism. The aim of this study was to use multiplex-PCR as an accurate, safe and rapid method to detect Brucella spp. and Leptospira spp. in aborted fetuses of bovine, ovine and caprine herds in Isfahan province (Iran). DNA was obtained directly from the stomach contents of aborted fetuses and PCR was performed by two pair novel primers. In total of the 276 specimens, 40 (14.4%) and 25 (9.0%) were identified positive for Brucella spp. and Leptospira spp., respectively. The convenience and the possibility of detection of both bacteria at a time, strongly support the use of this mPCR for routine diagnostics.
引用
收藏
页码：4627 / 4630
页数：4
相关论文
共 50 条
  • [31] Primers specific for detection of Myxococcus spp. by polymerase chain reaction
    Pepper, A. F.
    Martin, K. J.
    Bull, C. T.
    [J]. PHYTOPATHOLOGY, 2004, 94 (06) : S83 - S83
  • [32] Epidemiology and Detection of Bacterial Diarrhea Using Multiplex Polymerase Chain Reaction, Focusing on Campylobacter spp.
    Oh, Eun Ju
    Jang, Tae Su
    Park, Jung Min
    Kim, Jae Kyung
    [J]. American Journal of Biochemistry and Biotechnology, 2023, 19 (01): : 121 - 127
  • [33] Development of a multiplex polymerase chain reaction technique for detection and discrimination of Eimeria spp. in cattle in Indonesia
    Ekawasti, Fitrine
    Nurcahyo, Raden Wisnu
    Nashrulloh, Mukh Fajar
    Priyowidodo, Dwi
    Prastowo, Joko
    [J]. VETERINARY WORLD, 2022, 15 (04) : 975 - 980
  • [34] Development of a multiplex PCR for the identification of Staphylococcus spp. as a cause of caprine mastitis
    Ruiz-Romero, R. A.
    Cervantes-Olivares, R. A.
    Martinez-Gomez, D.
    Ducoing-Watty, A. E.
    Hernandez-Andrade, L.
    [J]. ARCHIVOS DE MEDICINA VETERINARIA, 2013, 45 (03) : 327 - 331
  • [35] Detection and differentiation of Listeria spp. by a single reaction based on multiplex PCR
    Bubert, A
    Hein, I
    Rauch, M
    Lehner, A
    Yoon, B
    Goebel, W
    Wagner, M
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1999, 65 (10) : 4688 - 4692
  • [36] Quantitative analysis of brucella spp in aborted bovine fetuses by real-time PCR
    Aslan, S.
    Yoldas, A.
    Yigin, A.
    Demirci, M.
    Saglam, F. Yarimcam
    [J]. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2016, 45 : 477 - 477
  • [37] Cross-sectional study on Brucella spp., Leptospira spp. and Salmonella spp. in bats from Montes Claros, Minas Gerais, Brazil
    Ferreira, Amanda Carvalho Rosado
    Vieira, Thallyta Maria
    Custodio, Dirceia Aparecida da costa
    Melo, Maria Norma
    Gontijo, Celia Maria Ferreira
    Lage, Andrey Pereira
    Dorneles, Elaine Maria Seles
    [J]. COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, 2021, 78
  • [38] MOLECULAR DETECTION OF SALMONELLA SPP. IN RAW CHICKEN MEAT IN THE ISFAHAN CITY, Iran BY POLYMERASE CHAIN REACTION (PCR) TECHNIQUE
    Khamesipour, Faham
    Khodadadi, Meysam
    Mamoreh, Morteza Azhdary
    [J]. IRANIAN JOURNAL OF PUBLIC HEALTH, 2014, 43 : 166 - 166
  • [39] Identification of Leptospira spp. carriers among seroreactive goats and sheep by polymerase chain reaction
    Lilenbaum, W.
    Varges, R.
    Ristow, P.
    Cortez, A.
    Souza, S. O.
    Richtzenhain, L. J.
    Vasconcellos, S. A.
    [J]. RESEARCH IN VETERINARY SCIENCE, 2009, 87 (01) : 16 - 19
  • [40] Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR
    Romero, C
    Lopez-Goñi, I
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1999, 65 (08) : 3735 - 3737
12345