Expression and localization of Kv1 potassium channels in rat dorsal and ventral spinal roots

被引：11

作者：

Utsunomiya, Iku ^{[1
]}

Yoshihashi, Elkichi ^{[1
]}

Tanabe, Shinya ^{[1
]}

Nakatani, Yoshihiko ^{[1
]}

Ikejlma, Hideaki ^{[1
]}

Miyatake, Tadashi ^{[2
]}

Hoshi, Keiko ^{[1
]}

Taguchi, Kyoji ^{[3
]}

机构：

[1] Showa Pharmaceut Univ, Dept Pharmacotherapeut, Tokyo 1948543, Japan

[2] Riken Frontier Res Syst, Wako, Saitama 3510198, Japan

[3] Showa Pharmaceut Univ, Dept Med Pharmacol, Tokyo 1948543, Japan

来源：

EXPERIMENTAL NEUROLOGY | 2008年 / 210卷 / 01期

关键词：

potassium channel; dorsal spinal root; ventral spinal root; juxtaparanodal region;

D O I：

10.1016/j.expneurol.2007.09.032

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We investigated the expression and localization of Kv1 channels in dorsal spinal roots (DRs) and ventral spinal roots (VRs) in rats. Among Kv1. 1-1.6 tested by RT-PCR, mRNAs of Kv 1.1, 1.2, and 1.5 were moderately expressed, those of Kv1.3 and Kv1.6 were weakly expressed, and that of Kv1.4 was hardly expressed at all in both DRs and VRs, whereas all six mRNAs were detected in spinal cord. Western blotting revealed that the major immunoreactive proteins were Kv1.1 and Kv1.2 in both DRs and VRs. Quantitative analysis indicated that levels of Kv1.1 and Kv 1.2 protein were significantly higher in DRs than VRs. Immunohistochemical examination showed that Kv 1.1 and Kv 1.2 were colocalized in juxtaparanodal regions of axons in both DRs and VRs. Finally, immunoprecipitation experiments revealed that Kv1.1 and Kv1.2 were coassembled. These findings indicate that Kv1 subtypes in DRs and VRs are somewhat different from those in spinal cord, and that the numbers of Kv1.1 and Kv1.2 channels are higher in DRs than VRs. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：51 / 58

页数：8

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