Single-step separation of lactate dehydrogenase using thiophilic chromatography

被引：0
|
作者：
Kminkova, M [1 ]
Kucera, J [1 ]
机构：
[1] Food Res Inst, CZ-10231 Prague 10, Czech Republic
来源：
COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS | 1998年 / 63卷 / 06期
关键词：
lactate dehydrogenase; carp; thiophilic chromatography; enzymes; oxidoreductases; Cyprinus carpio;
D O I：
10.1135/cccc19980851
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Lactate dehydrogenase (EC 1.1.1.27) from carp (Cyprinus carpio) hepatopancreas was purified by the single-step chromatography on thiophilic sorbent. Hydrophobic negative sorption was used as negative adsorption step for final purification. Final specific activity was 8.88 U/mg protein and the yield 84%. The enzyme is not adsorbed during hydrophobic interaction chromatography, but proteolytic activity is adsorbed completely on hydrophobic sorbent. Thus hydrophobic adsorption can be used as a prepurification step.
引用
收藏
页码：851 / 856
页数：6
相关论文
共 50 条
  • [41] Single-step separation of bioactive flavonol glucosides from Osteomeles schwerinae by high-speed counter-current chromatography
    Lee, Jun
    Jang, Dae Sik
    Yoo, Nam Hee
    Lee, Yun Mi
    Kim, Joo-Hwan
    Kim, Jin Sook
    JOURNAL OF SEPARATION SCIENCE, 2010, 33 (4-5) : 582 - 586
  • [42] Single-step vertical plasma separation of whole blood for tests and sample prep
    Alter, J
    GENETIC ENGINEERING NEWS, 1996, 16 (20): : 30 - 30
  • [43] A SINGLE-STEP PURIFICATION OF RAT PANCREATIC AND SALIVARY AMYLASE BY AFFINITY-CHROMATOGRAPHY
    BURRILL, PH
    BRANNON, PM
    KRETCHMER, N
    ANALYTICAL BIOCHEMISTRY, 1981, 117 (02) : 402 - 405
  • [44] SINGLE-STEP STABILITY STUDIES
    ERIKSEN, SP
    STELMACH, H
    JOURNAL OF PHARMACEUTICAL SCIENCES, 1965, 54 (07) : 1029 - &
  • [45] A single-step preparation of carbohydrate functionalized monoliths for separation and trapping of polar compounds
    Wang, Jincai
    Guo, Jialiang
    Chen, Haowen
    Huang, Xiaoling
    Somsen, Govert W.
    Song, Fenyun
    Jiang, Zhengjin
    JOURNAL OF CHROMATOGRAPHY A, 2020, 1628 (1628)
  • [46] Single-step strategy for reprocessing
    不详
    NUCLEAR ENGINEERING INTERNATIONAL, 2020, 65 (793): : 43 - 43
  • [47] Single-step synthesis of disiloxanetetraols
    Endo, Hisayuki
    Takeda, Nobuhiro
    Unno, Masafumi
    JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY, 2019, 89 (01) : 37 - 44
  • [48] Single-step synthesis of disiloxanetetraols
    Hisayuki Endo
    Nobuhiro Takeda
    Masafumi Unno
    Journal of Sol-Gel Science and Technology, 2019, 89 : 37 - 44
  • [49] Single-step preparation of nanocomposites
    Mathur, S
    Veith, M
    Shen, H
    Hüfner, S
    METASTABLE, MECHANICALLY ALLOYED AND NANOCRYSTALLINE MATERIALS, 2002, 386-3 : 341 - 346
  • [50] Separation of turkey lactate dehydrogenase isoenzymes using isoelectric focusing technique
    Heinova, Dagmar
    Kostecka, Zuzana
    Csank, Tomas
    ELECTROPHORESIS, 2016, 37 (02) : 335 - 338
12345