Single-step separation of lactate dehydrogenase using thiophilic chromatography

被引：0
|
作者：
Kminkova, M [1 ]
Kucera, J [1 ]
机构：
[1] Food Res Inst, CZ-10231 Prague 10, Czech Republic
来源：
COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS | 1998年 / 63卷 / 06期
关键词：
lactate dehydrogenase; carp; thiophilic chromatography; enzymes; oxidoreductases; Cyprinus carpio;
D O I：
10.1135/cccc19980851
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Lactate dehydrogenase (EC 1.1.1.27) from carp (Cyprinus carpio) hepatopancreas was purified by the single-step chromatography on thiophilic sorbent. Hydrophobic negative sorption was used as negative adsorption step for final purification. Final specific activity was 8.88 U/mg protein and the yield 84%. The enzyme is not adsorbed during hydrophobic interaction chromatography, but proteolytic activity is adsorbed completely on hydrophobic sorbent. Thus hydrophobic adsorption can be used as a prepurification step.
引用
收藏
页码：851 / 856
页数：6
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