MicroRNA-383-5p inhibits the proliferation and promotes the apoptosis of gastric cancer cells by targeting cancerous inhibitor of PP2A

The aberrant expression of microRNA (miRNAor miR)-383-5p has been found in numerous types of cancer. However, the function of miR-383-5p in gastric cancer (GC) remains elusive and requires further investigation. In the present study, the level of miR-383-5p and cancerous inhibitor of PP2A (CIP2A) in GC cell lines was determined by reverse transcription-quantitative PCR analysis. GC cell proliferation, apoptosis and cell cycle distribution were determined by the MTT assay and flow cytometry, respectively. The mRNA target of miR-383-5p was identified by dual luciferase activity assay. It was observed that the expression of miR-383-5p was lower and that of CIP2A was higher in GC cells compared with the GES-1 normal human gastric epithelial cell line. Transfectoin with miR-383-5p mimic significantly inhibited GC cell proliferation, while it promoted cell apoptosis and G(0)/G(1) arrest by targeting CIP2A. Taken together, the findings of the present study demonstrate that miR-383-5p inhibits GC cell proliferation and promotes apoptosis and G(0)/G(1) arrest by targeting CIP2A, indicating that targeting miR-383-5p may hold promise as a future therapeutic strategy for patients with GC.