Evaluation of carbon isotope fractionation during anaerobic reductive dehalogenation of chlorinated and brominated benzenes

被引:8
|
作者
Sohn, Seo Yean [1 ]
Kuntze, Kevin [2 ]
Nijenhuis, Ivonne [2 ]
Haeggblom, Max M. [1 ]
机构
[1] Rutgers State Univ, Sch Environm & Biol Sci, Dept Biochem & Microbiol, 76 Lipman Dr, New Brunswick, NJ 08901 USA
[2] UFZ Helmholtz Ctr Environm Res, Dept Isotope Biogeochem, Permoserstr 15, D-04318 Leipzig, Germany
基金
欧盟第七框架计划; 美国食品与农业研究所;
关键词
Compound specific carbon isotope analysis; Halogenated benzenes; Microbial reductive dehalogenation; Anaerobic; Aquatic sediments; MIXED CULTURE; MICROBIAL CONSORTIUM; STRAIN CBDB1; DECHLORINATION; HEXACHLOROBENZENE; MONOCHLOROBENZENE; BIODEGRADATION; TRANSFORMATION; TETRACHLOROETHENE; DEGRADATION;
D O I
10.1016/j.chemosphere.2017.11.089
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Compound specific stable isotope analysis (CSIA) has been established as a useful tool to evaluate in situ biodegradation. Here, CSIA was used to determine microbial dehalogenation of chloro- and bromobenzenes in microcosms derived from Hackensack River sediments. Gas chromatography-isotope ratio mass spectrometry (GC-IRMS) was used to measure carbon isotope fractionation during reductive dehalogenation of hexachlorobenzene (HCB), pentachlorobenzene (PeCB), 1,2,3,5-tetrachlorobenzene (TeCB), 1,2,3,5-tetrabromobenzene (TeBB), and 1,3,5-tribromobenzene (TriBB). Strong evidence of isotope fractionation coupled to dehalogenation was not observed in the substrate, possibly due to the low solubilities of the highly halogenated benzene substrates and a dilution of the isotope signal. Nonetheless, we could measure a depletion of the delta C-13 value in the dichlorobenzene product during dechlorination of HCB, the sequential depletion and enrichment of delta C-13 value for trichlorobenzene in TeCB dechlorinating cultures, and the enrichment of delta C-13 during debromination of TriBB. This indicates that a measurable isotope fractionation occurred during reductive dehalogenation of highly halogenated chloro- and bromobenzenes in aquatic sediments. Thus, although more quantitative measurements will be needed, the data suggests that CSIA may have application for monitoring in situ microbial reductive dehalogenation of highly halogenated benzenes. (C) 2017 Published by Elsevier Ltd.
引用
收藏
页码:785 / 792
页数:8
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