Lectin activity of the nucleocytoplasmic EUL protein from Arabidopsis thaliana

被引：28
|
作者：
Van Hove, Jonas [1 ]
Fouquaert, Elke [1 ]
Smith, David F. [2 ]
Proost, Paul [3 ]
Van Damme, Els J. M. [1 ]
机构：
[1] Univ Ghent, Lab Biochem & Glycobiol, Dept Mol Biotechnol, B-9000 Ghent, Belgium
[2] Emory Univ, Sch Med, Dept Biochem, Atlanta, GA 30322 USA
[3] Katholieke Univ Leuven, Lab Mol Immunol, Rega Inst Med Res, B-3000 Louvain, Belgium
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2011年 / 414卷 / 01期
关键词：
Carbohydrate binding; EUL domain; Lectin; Localization; Nucleus; Plant protein; CDNA; GENE; AGGLUTININ; BINDING; CLONING; STRESS; FAMILY;
D O I：
10.1016/j.bbrc.2011.09.031
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Euonymus lectin (EUL) domain was recognized as the structural motif for a novel class of putative carbohydrate binding proteins. Confocal microscopy demonstrated that the lectin from Euonymus europaeus (EEA) as well as the EUL protein from Arabidopsis thaliana (ArathEULS3) are located in the nucleocytoplasmic compartment of the plant cell. ArathEULS3 as well as its EUL domain were successfully expressed in Pichia pastoris and purified. The EUL domain from Arabidopsis interacts with glycan structures containing Lewis Y, Lewis X and lactosamine, indicating that it can be considered a true lectin domain. Despite the high sequence identity between the EUL domains in EEA and ArathEULS3, both domains recognize different carbohydrate structures. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：101 / 105
页数：5
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