Optimized automated solid phase synthesis of oligonucleotides and derivatives

被引：0
|
作者：
Urbina, GA
Grübler, G
Weiler, A
Echner, H
Stoeva, S
Schernthaner, J
Gross, W
Voelter, W
机构：
[1] Univ Tubingen, Inst Physiol Chem, Phys Biochem Abt, D-72076 Tubingen, Germany
[2] Eppendorf Netheler Hinz GmbH, Biochem & Mol Grp, D-22339 Hamburg, Germany
来源：
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION B-A JOURNAL OF CHEMICAL SCIENCES | 1998年 / 53卷 / 09期
关键词：
oligonucleotides; thio-oligonucleotides; 5 ' and 3 '-derivatisation of oligonucleotides; automatic oligonucleotide synthesis; gene assembly;
D O I：
暂无
中图分类号：
O61 [无机化学];
学科分类号：
070301 ; 081704 ;
摘要：
An optimized automated synthesizer is presented for assembling oligonucleotides, thio-oligonucleotides and 5'-modified oligonucleotides including: chemical phosphorylation, multihydroxyl derivatization with a non-nucleosidic phosphoramidite. The incorporation of biotin, fluorescein and rhodamine phosphoramidites is described. The purification and structure determination of oligo-nucleotides was confirmed using high performance liquid chromatography (HPLC), capillary electrophoresis (CE) and laser desorption mass spectrometry (LDMS). Several applications and confirming data will be presented for gene synthesis and polymerase chain reaction (PCR) experiments.
引用
收藏
页码：1051 / 1068
页数：18
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