Determination of ketorolac in human serum by square wave adsorptive stripping voltammetry

The adsorption behavior of ketorolac on a hanging mercury drop electrode (HMDE) was explored by square-wave and cyclic voltammetry. The square wave voltammetric response of ketorolac depends on the parameters of the square wave voltammetry excitation signal as well as on the pH of the medium and the accumulation time. The drug was accumulated at HMDE and a well-defined peak was obtained at - 1.41 V versus. Ag/AgCl (saturated KCI) in acetate buffer of pH 5.0. A square-wave adsorptive stripping voltammetric method for the quantitative determination of ketorolac was developed. The linear concentration range was 1 x 10(-10)-1 x 10(-8) when using 300 s accumulation at - 0.8 V. The detection limit of ketorolac was 1.0 x 10(-11) M. The precision was excellent with relative standard deviation of 3.85% at concentration of 5 x 10(-8) M after 60 s accumulation time. Applicability to serum samples was illustrated. A detection limit of 14 ng per mi of serum was obtained. (C) 2001 Elsevier Science B.V. All rights reserved.