Determination of nifuroxazide in human serum by adsorptive stripping voltammetry

The adsorption behavior of nifuroxazide on a hanging mercury drop electrode (HMDE) was critically examined using cyclic voltammetry and differential pulse techniques. The effect of different parameters on the accumulation behavior of the adsorbed species was tested. Thus, a sensitive stripping voltammetry procedure for the determination of nifuroxazide with an adsorptive accumulation on the surface of HMDE has been developed. Under optimal conditions a detection limit of 5x10(-9) M and a linear calibration graph in the range 2x10(-8) - 1x10(-7) M were obtained. The precision, expressed by the relative standard deviation, was 2.8% (n=10) at 1x10(-7) M. The method was successfully applied for the analysis of nifuroxazide in serum.