Lineage-Specific Real-Time Reverse Transcription PCR for Yellow Fever Virus Outbreak Surveillance, Brazil

被引：27

作者：

Fischer, Carlo ^{[1
,2
]}

Torres, Maria C. ^{[3
]}

Patel, Pranav ^{[4
]}

Moreira-Soto, Andres ^{[1
,5
,6
,7
,8
]}

Gould, Ernest A. ^{[9
]}

Charrel, Remi N. ^{[9
,10
]}

de Lamballerie, Xavier ^{[9
,10
]}

Ribeiro Nogueira, Rita Maria ^{[3
]}

Sequeira, Patricia C. ^{[3
]}

Rodrigues, Cintia D. S. ^{[3
]}

Kummerer, Beate M. ^{[1
]}

Drosten, Christian ^{[2
,5
,6
,7
,8
]}

Landt, Olfert ^{[4
]}

Bispo de Filippis, Ana Maria ^{[3
]}

Drexler, Jan Felix ^{[2
,5
,6
,7
,8
]}

机构：

[1] Univ Bonn, Med Ctr, Bonn, Germany

[2] German Ctr Infect Res DZIF, Tubingen, Germany

[3] Inst Oswaldo Cruz, Rio De Janeiro, Brazil

[4] TIB MOLBIOL Syntheselabor GmbH, Berlin, Germany

[5] Charite Univ Med Berlin, Berlin, Germany

[6] Free Univ Berlin, Berlin, Germany

[7] Humboldt Univ, Berlin, Germany

[8] Berlin Inst Hlth, Inst Virol, Berlin, Germany

[9] Aix Marseille Univ, Marseille, France

[10] Inst Hosp Univ Mediterranee Infect, Marseille, France

来源：

EMERGING INFECTIOUS DISEASES | 2017年 / 23卷 / 11期

关键词：

VACCINE; IMMUNOGENICITY; DISEASE;

D O I：

10.3201/eid2311.171131

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between vaccine and American wild-type YFV. We validated these highly specific and sensitive assays in an outbreak setting.

引用

页码：1867 / 1871

页数：5

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