Strategies for prokaryotic expression of eukaryotic membrane proteins

被引：45
|
作者：
Laage, R [1 ]
Langosch, D [1 ]
机构：
[1] Heidelberg Univ, Dept Neurol, D-69120 Heidelberg, Germany
来源：
TRAFFIC | 2001年 / 2卷 / 02期
关键词：
expression; synaptobrevin; syntaxin; SNARE; transmembrane segment; E; coli;
D O I：
10.1034/j.1600-0854.2001.020204.x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
High-level heterologous expression of integral membrane proteins at full-length is a useful tool for their structural and functional characterization. Here, systems that have previously been used for efficient bacterial expression of eukaryotic membrane proteins are reviewed and novel vectors consisting of a modular fusion moiety based on nuclease A from Staphylococcos aureus are presented.
引用
收藏
页码：99 / 104
页数：6
相关论文
共 50 条
  • [41] Enhanced regulation of prokaryotic gene expression by a eukaryotic transcriptional activator
    MacDonald, I. Cody
    Seamons, Travis R.
    Emmons, Jonathan C.
    Javdan, Shwan B.
    Deans, Tara L.
    NATURE COMMUNICATIONS, 2021, 12 (01)
  • [42] Enhanced regulation of prokaryotic gene expression by a eukaryotic transcriptional activator
    I. Cody MacDonald
    Travis R. Seamons
    Jonathan C. Emmons
    Shwan B. Javdan
    Tara L. Deans
    Nature Communications, 12
  • [43] Bifunctional reporter genes: construction and expression in prokaryotic and eukaryotic cells
    Goldenkova, IV
    Musiychuk, KA
    Piruzian, ES
    MOLECULAR BIOLOGY, 2003, 37 (02) : 307 - 313
  • [44] PROKARYOTIC AND EUKARYOTIC REPLICONS
    HUBERMAN, JA
    CELL, 1995, 82 (04) : 535 - 542
  • [45] CONSTRUCTION OF A NEOFUSION GENE FOR EXPRESSION IN BOTH PROKARYOTIC AND EUKARYOTIC CELLS
    HARWOOD, AJ
    SHERVINGTON, A
    BOSTOCK, CJ
    GENE, 1990, 88 (02) : 279 - 283
  • [46] Prokaryotic membrane coat - like proteins: An update
    Ferrelli, Leticia
    Pidre, Matias L.
    Garcia-Dominguez, Ruben
    Alberca, Lucas N.
    del Saz-Navarro, DMaria
    Santana-Molina, Carlos
    Devos, Damien P.
    JOURNAL OF STRUCTURAL BIOLOGY, 2023, 215 (03)
  • [47] Comparative analysis and "expression space'' coverage of the production of prokaryotic membrane proteins for structural genomics
    Surade, Sachin
    Klein, Markus
    Stolt-Bergner, Peggy C.
    Muenke, Cornelia
    Roy, Ankita
    Michel, Hartmut
    PROTEIN SCIENCE, 2006, 15 (09) : 2178 - 2189
  • [48] Prokaryotic structural maintenance of chromosomes (SMC) proteins: distribution, phylogeny, and comparison with MukBs and additional prokaryotic and eukaryotic coiled-coil proteins
    Soppa, J
    GENE, 2001, 278 (1-2) : 253 - 264
  • [49] The N-end rule pathway for regulated proteolysis: prokaryotic and eukaryotic strategies
    Mogk, Axel
    Schmidt, Ronny
    Bukau, Bernd
    TRENDS IN CELL BIOLOGY, 2007, 17 (04) : 165 - 172
  • [50] Genetic analysis of prokaryotic and eukaryotic DNA-binding proteins in Escherichia coli
    Whipple, FW
    NUCLEIC ACIDS RESEARCH, 1998, 26 (16) : 3700 - 3706
12345