A high-throughput screening method for endophytic bacteria with antagonistic activity against Magnaporthe oryzae in rice (Oryza sativa L.) seeds

被引：2
|
作者：
Wang, Zhishan [1 ]
Wu, Xianyu [1 ]
Li, Ni [2 ]
Wang, Weiping [2 ]
Liu, Yang [1 ]
机构：
[1] Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Beijing 100083, Peoples R China
[2] Hunan Hybrid Rice Res Ctr, State Key Lab Hybrid Rice, Changsha 410125, Peoples R China
来源：
PLANT GROWTH REGULATION | 2022年 / 96卷 / 02期
关键词：
Rice seed; Endophytes; High-throughput screening; Antifungal activity; Biological control; ZEA-MAYS L; COMMUNITY; DIVERSITY; STRAIN;
D O I：
10.1007/s10725-021-00771-z
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Screening for microorganisms with antagonistic activity against crop pathogens is essential for microbial antagonist preparation, crop disease control, and sustainable agricultural development. This study established a rapid, efficient, one-step high-throughput method for screening endophytic bacteria displaying antagonistic activity against Magnaporthe oryzae (M. oryzae) ACCC36020 in rice seeds. The experimental results indicated that these bacteria could be directly screened from six sample groups using this method. This technique can achieve simultaneous screening and purification, identify endophytic bacteria displaying antifungal activity in rice seeds, and save significant time.
引用
收藏
页码：237 / 241
页数：5
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