Regulation of osteogenic differentiation of bone marrow mesenchymal stem cells by intermittent hypoxia

被引：0
|
作者：
Wei, Silong [1 ,2 ,3 ,4 ]
Guo, Xiaojing [5 ]
Lu, Xiaofeng [1 ,2 ,3 ,4 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Dept Oral & Craniomaxillofacial Surg, Shanghai, Peoples R China
[2] Shanghai Key Lab Stomatol, Shanghai, Peoples R China
[3] Shanghai Res Inst Stomatol, Shanghai, Peoples R China
[4] Natl Clin Res Ctr Stomatol, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China
[5] Fudan Univ, Shanghai Stomatol Hosp, Oral Biomed Engn Lab, Shanghai, Peoples R China
来源：
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE | 2018年 / 11卷 / 11期
关键词：
Intermittent hypoxia; bone marrow mesenchymal stem cells; osteogenic differentiation; obstructive sleep-apnea hypopnea syndrome; OBSTRUCTIVE SLEEP-APNEA; MINERAL DENSITY; RAT MODEL;
D O I：
暂无
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Objective: The aim of this study was to explore the effects of intermittent hypoxia on osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Methods: Primary culturing of rat BMSCs was conducted by adherent cell culturing. BMSCs were identified with immunofluorescence staining. Third-generation BMSCs were randomly divided into the control group and intermittent hypoxia group. The two groups were compared in the formation of mineralized bone nodules after 3 weeks of culture and Real-time PCR was performed for testing the mRNA levels of the bone differentiation markers (ALP, Col-1 and BMP-2) in the two groups at 24 h, 48 h and 72 h, respectively. Results: BMSCs were successfully cultured in this trial. Significantly more mineralized nodules of BMSCs, at different time points, were observed in the intermittent hypoxia group than the control group (P<0.001). Moreover, mRNA levels of ALP, Col-1, and BMP-2 in the intermittent hypoxic group were significantly higher than those in the control group (all P<0.01). Conclusion: Intermittent hypoxia improves osteogenic differentiation of BMSCs.
引用
收藏
页码：11995 / 12001
页数：7
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